基于HPLC-MS/MS的肉类喹诺酮残留抗干扰检测方法研究

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Abstract： This paper establishes an eficient detection method for quinolone veterinary drug residues in meat based on the High Performance Liquid Chromatography-Tandem Mass Spectrometry （HPLC-MS/MS）analysis system.The matrix interference was reduced by adopting the acidified aetonitrile extraction combined with enhanced lipid removal and purification strategy，and the dynamic multi-reaction monitoring parameters were optimized to improve the detection performance.The results showed that the five quinolones had a good linear relationship in the range of 0.1mg.L-1 to 100.0mg-1-1 ，with correlation coefcients （R2） all greater than 0.995.The detection limits were in the range of to 0.30μg⋅kg-1 ， and the quantification limits were in the range of 0.1μg⋅kg-1 to 1.0μg⋅kg-1 At the three spiked levels of low，medium and high，the spiked recoveryrate of the target substance was 82.1% to 109.1% ， therelative standard deviation was 0.70% to 8.20% ， and the matrix effect was controlled within ±15% The actual positive rate of sample detection was 4.2% ，and non-target metabolites could be traced. This method is sensitive and acurate，and can provide technical support for the supervision and safety risk research of quinolone residues in meat.

Keywords： High Performance Liquid Chromatography-Tandem Mass Spectrometry （HPLC-MS/MS）; quinolones veterinary drugs; matrix effect

喹诺酮类兽药作为广谱抗菌剂在畜牧业中广泛应用，致使其在动物源性食品中的残留问题愈发突出。（剩余6404字）