低温乳制品中金黄色葡萄球菌实时荧光PCR检测方法的建立和应用

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Abstract： In this paper，a real-time fluorescent Polymerase Chain Reaction （PCR） rapid detection method system for Staphylococcus aureus was established fortherapid release of low-temperature dairy products.It can complete enrichment and rapid detection within 24h Moreover， internal standard gene amplification was added in thereal-time fluorescent PCR reaction to monitor whether the PCR amplification reaction was inhibited.Specificity， sensitivityand consistency with themethodof National Standard for Food Safety-Microbiological Examination of Food-Detection of Staphylococcus aureus （GB 4789.10—2016） were validated according to the requirement of ISO 16140-2：2016，and sample application tests are carried out in dairy factories. the results showed that the method has good specificity，the detection limit is1CFU/25g（mL），Theresultsof artificial contaminationsample andactual sample testing showed thatthe method was in good consistency with GB 4789.10—2016 in several low-temperature dairy products， with a relative accuracy of 100% .In addition，when the method results were negative，the internal control results were normal， which played a role in monitoring the reaction process.In summary，the method in this study can be used for the rapid qualitative detection of Staphylococcus aureus in low-temperature dairy products.

Keywords： real-time fluorescence Polymerase Chain Reaction （PCR）; internal control; low-temperature dair products; Staphylococcus aureus

金黄色葡萄球菌是乳制品质量安全的主要风险因子之一。（剩余6461字）