纳米酶抑菌膜制备及其功效评估的实验教学设计

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中图分类号：TS207.3 文献标志码：A 文章编号：1674-0270（2025）35-0165-03

Abstract： Basedon the scientificresearchachievements of novel nanozymes withantibacterialactivityandthereform needs offood microbiology experimental teaching，this paper explores the design of a comprehensive microbiology experiment utilizing nanozyme-based antibacterial films.Building onthe previously constructed FeMOF nanozyme with peroxidase-like activity，FeMOF/Chitosan films are developed by combining it with biodegradable chitosan. Using E .coli as the experimental strain，the antibacterial performance of the hybrid film isevaluated through methods including culture medium preparation，bacterial activation，turbidimetry，and plate counting.This paper aims to enable undergraduate students to master the preparation of novelantibacterial nanozyme materials and the methods for evaluating antibacterial performance.

Key Words： nanozyme; antibacterial fim; antibacterial eficacyevaluation; microbiology experiment; instructional design

食品微生物学实验是检验学生对理论知识理解和运用的重要途径。（剩余4064字）