高效液相色谱-柱后衍生测定粮食中黄曲霉毒素 B1 含量
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中图分类号：TS201.6 文献标志码：A 文章编号：1674-0270（2025）32-0105-03
Determination of Aflatoxin B1 in CerealsbyHPLC with Post-Column Derivatization
XI Yunfei，WANGXu，LIYundan，ZHANG Guirong，QIN Shujun （Fanshi County ComprehensiveInspectionand Testing Center，Xinzhou O343oo，China
Abstract：This article establishesa rapidand eficient high-performance liquid chromatography method for the determination ofaflatoxinI β1 in cereals.Samples were extracted with a methanol and water mixture at a volume ratio of 70：30 and then the extract was purified byaspecific immunoaffinity column for aflatoxin B -1 .In the elution step，methanol and water at a volume ratio of 1：1 were usedas the eluent，followed by detection with afluorescence detector combined with photochemical post-column derivatization. The results show that aflatoxin B1 has a good linear relationship in the concentration range of （20 0.1～40.0ng/mL with thecorrelation coefficient reaching O.999 6.The average recoveries of the method are inthe range of 92.4% 100% ，and the relative standard deviationsare between 2.0% （ 2.7% . The limit of quantification is determined to be 0.068μg/kg .All performance indicators meet the relevant detection standards.
Key Words：aflatoxin B1; high-performance liquid chromatography with post-columnderivatization; cereals
黄曲霉毒素主要源自黄曲霉及集蜂曲霉等特定真菌的代谢过程[]，具有很强的致癌性[2、致畸性、致突变性，主要通过扰乱DNA和RNA来干扰蛋白质合成，导致人体全身性损害[3。（剩余3723字）