基于免疫磁珠技术的金黄色葡萄球菌快速检测技术研究

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中图分类号：TS201.3 文献标志码：A 文章编号：1674-0270（2025）32-0072-03

Research on Rapid Detection Technology for Staphylococcus aureus Based on Immunomagnetic Bead Technology

MA Jie (Qingxian DiseasePrevention and Control Center, Cangzhou O6265O, China)

Abstract:To enhance the rapid identificationof Staphylococcus aureus incomplex food matrices,a detection technology system centeredon specificcaptureof immunomagnetic beads is established.The studyanalyzes the beadbacterium binding behavior,theimpactof sample pretreatmentconditionsoncapture eficiency,andthesignalreadout mechanismunderlow-relativeabundance conditions.Results indicate thatthe functionalization level of magnetic beads,ionicenvironmentstability,and microstructureconfinementdesigncollectivelydeterminethedetectionsystem's sensitivity.Theoptimized strategy basedonlayered amplificationand local enrichment significantly enhances the detectabilityoflow-copycteria,omprehensivelyimproving thtimeliness,accuracy,andenarioadaptabilityofpid detection.Thispaperprovidesanengineredandscalable technical foundationforfood microbiologicalsafety monitoring. Key Words:immunomagnetic beads; Staphylococcus aureus; rapid detection

金黄色葡萄球菌作为食品加工链条中常见的致病微生物之一，早期污染量低、分布离散、易受复杂基质屏蔽。（剩余5067字）