复合水凝胶仿生细胞外基质构建的3D模型用于化疗研究

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中图分类号： TQ427.2+6 文献标志码：A

文章编号：1001-5922（2026）1-0051-05

Abstract：Gelatin methacrylate （GelMA）was prepared using gelatin and methacrylic anhydride.Different mass concentrations of polyethylene glycoldiacrylate（PEGDA）wereadded，followed bymixingand photocrossinking topreparegelatinmethacrylate-polyethylene glycoldiacrylatecompositehydrogels（GelMA-PEGDAhydrogels）with diferentstifneses.These hydrogels were used to bionicall construct 3D breast cancer models of extracelular matrices with different stifnesses，soastoinvestigatetheeffectsofextracellularmatrices withdiferentstiffnessesonthegrowthofbreastcancer cells MDA - MB -231 and MCF -7 and their sensitivity to chemotherapeutic drugs paclitaxel and doxorubicin.Nuclear magneticresonancespectroscopycharacterizationshowed thatthegrafting rateof gelatinand methacrylicanhydridewas （2号 84.38% ;Fourier transform infrared spectroscopy characterization confirmed that GelMA was mixed with diffrent mass concentrationsofPEGDAand then photocrosslinked to form（5G1P，5G3P）hydrogel crossinks.Dynamic thermomechanical analyzerwas used to characterize the stiffess of hydrogels.The weighing method was used to characterize hydrogel swelling，and the massloss method was used to characterize the hydrogel degradationrate.Scanning electron microscopy wasusedtocharacterizethemorphologyofhydrogels.Live/dead staining wasusedtocharacterizethecytocompatibilityof hydrogels ，and the CCK -8 assay was used to evaluate the sensitivity to chemotherapeutic drugs.

Key words：gelatin;polyethylene glycol diacrylate;breast cancercels;chemotherapeutic drug sensitivity

肿瘤微环境（TME）对肿瘤的生长、侵袭和转移具有重要作用[1]。（剩余7942字）