基于CarbonS技术的UPLC-MS/MS法测定药酒中3种茛烷类生物碱

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Determination of Three Hyoscypane Alkaloids in Medicinal Wine by UPLC-MS/MS Method Based on Carbon S Technology

ZHONG Xue，MENG Chunyang， WU Yutian， ZHOU Yibing，LIN Ye， ZOU Lu* （Guizhou Provincial Center for Disease Control and Prevention， Guiyang 55ooo4， China） Abstract： Objective： To establish a rapid method for the detection of three types of hyoscypane alkaloids in medicinal wine.Method： The samples were extracted with acetonitrile and purified with Carbon S adsorbent.The mobile phase was methanol-5 mmol L-1 ammonium formate （containing 0.1% formic acid），and the detection was caried out in the multi-reaction monitoring positive ion mode.Result： The three kinds of hyoscypane alkaloids had a good linear relationship in the range of 2.0μg⋅L-1 to 100.0μg⋅L-1， and the correlation coefficient was greater than 0.999; the detection limit was 0.10μg⋅L-1 to 0.25μg⋅L-1 ，and the quantification limit was 0.25μg⋅L-1 to 1.00μg⋅L-1 the recovery rate of standard addition was 70.23% to 90.10% ，and the relative standard deviation was 1.71% to 7.44% Conclusion： This method is simple and accurate，and can be used for the rapid analysis of hyoscypane alkaloids in medicinal wine.

Keywords： hyoscypane alkaloids; medicinal liquor; Carbon S

茛蓉碱是一类广泛存在于茄科植物中的酯类化合物[]，多存在于曼陀罗属植物如洋金花中[2]。（剩余4580字）