高效液相色谱-串联质谱法测定食品中O-丙基伐地那非和O-丙基羟基伐地那非

打开文本图片集

Determination of O-Propyl Vardenafil and O-Hydroxypropyl Vardenafil in Foods by High Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHANG Sisi， ZHOU Xueyun （GuangdongHongke Testing Technology Co.，Ltd.，Huizhou 516ooo， China）

Abstract： To establish a method for the simultaneous determination of O-propyl vardenafil and O-hydroxypropyl vardenafil in food by high performance liquid chromatography-tandem mass spectrometry. Method： After ultrasonic extraction and constant volume with methanol aqueous solution， O-propyl vardenafil and O-hydroxypropyl vardenafil were separated under gradient elution conditions using 0.1% formic acid aqueous solution-acetonitrile as the mobile phase，and determined by mass spectrometry scanning with multiple reaction ion monitoring.Result： The two compounds had good linear relationships in the mass concentration range of 1ng⋅mL-1 to 100ng⋅mL-1 and 2ng⋅mL-1 （2 to 200ng⋅mL-1 ， and the correlation coefficient was greater than 0.990; the detection limit of O-propyl vardenafil was （20 0.05mg⋅kg-1 ，and the quantification limit was 0.10mg.kg-1 ; the detection limit of O-hydroxypropyl vardenafil was 0.10mg.kg-1 ，and the quantification limit was 0.20mg.kg-1 ；the recoveries at different addition levels were 83.4% to 103.0% ，and the relative standard deviation was less than 10% .All key indicators met the requirements for trace detection ofillegal aditives. Conclusion： The detection method constructed in this studyhas the advantages of simple operation，accuratequantitativedetermination and high sensitivity.It can simultaneously complete the determination of two new types of banned aditives，and can efectively meet the needs of screening，confirmation and quantitative analysis of banned additives in food.

Keywords： O-propyl vardenafil; O-hydroxypropyl vardenafil; illegal additives; foods

近年来，随着工作压力的不断增加，性功能障碍的发病率逐年上升，患者人数日益增多。（剩余4330字）