高效液相色谱法测定口香糖维生素E方法的优化

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Optimization of High Performance Liquid Chromatography for Determination of Vitamin E in Chewing Gum

CHEN Yongju （Guangzhou Inspection， Testing and Certification Group Co.，Ltd.， Guangzhou 511447， China）

Abstract： This article uses HPLC to determine the content of vitamin E in chewing gum，and studies the optimization of the detection method. Using a C30 chromatographic column for separation， methanol water was used as the mobile phase， with a flow rate of 0.5mL⋅min-1 and a column temperature of 25∘C .Fluorescence detection was performed at a wavelength of 294nm using an external standard method for quantitative analysis. The results showed thatvitamin E exhibited good linearity in the range of 0.25～10.00μg⋅mL-1 ，with a correlation coefficient of R2>0.999 . The detection limit was 14.0μg/100g ， the quantification limit was 48.0μg/100g ， the recovery rate was between 86.6% and 101.2% ，and the relative standard deviation not exceed 4.96% . This method is simple to test， highly sensitive，and suitable for the determination of vitamin E in chewing gum.

Keywords： chewing gum; vitamin E; high performance liquid chromatography

维生素E主要有 a 1 β 、 γ 和δ这4种形式，在促进人体生长发育、增强机体免疫力等方面发挥着重要作用[1-3]。（剩余3805字）