高效液相色谱法同时测定不同品种菊花中4种活性成分的含量

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Determination of Four Active Components in Different Chrysanthemum Varieties by High Performance Liquid Chromatography

DANG Yuxia （Traditional Chinese Medicine， Gulang County People's Hospitale， Gulang 733100， China）

Abstract： Objective： To establish a method for the simultaneous determination of the contents of four active components in chrysanthemums by high performance liquid chromatography， which isused to detect thecontents of the four active components in diferent varieties of chrysanthemums.Method： The gradient elution method is adopted. The flow rate is 1.0mL⋅min-1 ，the column temperature is 35∘C ，the detection wavelength is 348nm ，and quantification was performed by the external standard method.Result： The four components were well separated within 25min ，witharecoveryrateof 85.50% to 100.38% ，arelative standard deviationof 1.3% to 4.6% and the detection limit and quantification limit met the requirements.The contents of chlorogenic acid，luteolin， isochlorogenic acid B and isochlorogenic acid A in the five kinds of chrysanthemums were 0.304% to 0.775% 0.089% （2号 to 0.789% 0.088% to 0.341% and 0.621% to 1.981% ，respectively. Conclusion： This method is accurate and stable， and can provide a reference for evaluating the quality of different varieties of chrysanthemums.

Keywords： high performance liquid chromatography; chrysanthemum; active ingredient

1材料与方法

菊花作为常用的药食同源食品，具有清热去火、消炎解毒、平肝明目、抗炎抗病毒、抗氧化、降血糖血脂的功效，通常用于泡茶饮用或作为药用辅助，以改善口腔溃疡引起的疼痛、眼晴干涩红肿等不适症状[1-5]。（剩余2992字）