密闭环境危害因素所致睡眠障碍小鼠模型的构建

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AbstractObjective：To establishamouse modelofslep disorders bysimulatingconditions inaconfined environment.Methods：A total of 4O KM male mice were randomly divided into four groups（ n=10 each）：control group（CON group），light change group（Lgroup），restraint group（R group）and light change plus restraint group（LR group）.After30days of intervention，mice were subjectedtoasodiumpentobarbital-inducedsleeptest，inwhichsleep latencyandsleepdurationwererecorded.Hypothalamic tissesandserumsampleswere takenforthe measurementof slep-related neurotransmitersorhormones.The enzyme-linked immunosorbent assay（ELISA）was used to detect γ -aminobutyric acid（GABA）dopamine（DA），and 5- hydroxytryptophan（5-HT）inthehypothalamustisues.Serumhormones，includingorexinA（OXA），corticotropin（CO）corcotropin-releasing hormone（CRH）andadrenocorticotropic hormone（ACTH）werealso measured toevaluate themodeling ffects underdiffrentconditions.Results：ThebodyweightsoftheRandLRgroupsattheendof theexperimentweresignificantlylower （ P<0.05 ）compared withthe CON group. Under light change plus restraint condition，sleep latency was significantly prolonged （ P<0.05 ） and sleep duration was significantly shortened（ P<0.01 ） in mice. In the measurement of hypothalamus-related neurotransmitercontents，comparedwith theCONgroup，the mice intheLR grouphadsignificantlylowerGABAand 5-HTlevels （ P<0.05 ）and higherDA levels（ P<0.05， ）；in the measurement of serum-related hormone contents，compared with the CON group，the LR group had significantly higher OXA，CORT and ACTH levels（ P<0.05 for each）and CRH levels did not change significantly（ P>0.05 ）.Conclusion：Light plus restraint treatment in a confined environment can affect the levels of relevant neurotransmitersandhormones inthe hypothalamusandserum，disruptnormalsleepprocess inmice，andestablishamodelof sleep disorders.

KeywordsMice；Confined environment；Light；Restraint; Sleep disorder model；Hypothalamicneurotransmiters；Serum hormone;ELISA

中图分类号：R338.63 文献标识码：A doi：10.3969/j. issn.2095-7130.2025.07.002

密闭环境是指与外界相对隔离、进出受到严格限制的有限空间环境[1-2]。（剩余7636字）