肝郁脾虚方对失眠大鼠下丘脑 γ -氨基丁酸 和谷氨酸含量的影响

打开文本图片集

AbstractObjective：This study aims to investigate the eects of Ganyu Pixu Formula（GYPXF） on γ -aminobutyric acid（GABA）and Glutamate（Glu）levelsinthehypothalamusof insomniarats.Methods：Thirty-twospecificpathogen-fre（SPF）-grade male Sprague Dawley（SD）rats wererandomly divided into four groups，with 8rats ineach group：normal control group，model controlgroup，GYPXFgroup，anddazepamgroup.Exceptforthoseinthenoralcontrolgroup，allratsweresubjectedtotheodelingforliverdepresio-speedeficncyisomiasdromebhonictail-capngstiulationintraperitonealp-loylalanine（PCPA）injections andirregular feeding pattems（fastingonodd-numbered days withad libitum wateraccess）.After7 daysof intragastricadministrationwithcorresponding treatments，hypothalamicGABAandGlulevelswerequantifiedusingenzyme-linked immunosorbent assay（ELISA），with subsequentcalculationof Glu/GABAratios.Results：Compared with thenormal controlgroup，rats inthemodel groupexhibitedmanifestationsoflethargyandcoarse，lustreless fur，andsignificantlydecreased body weight（ P<0.01）. The hypothalamic GABA was significantly lower（ P<0.05） ，while Glu level was significantly higher in the model group than the normal control group（ P<0.01 ），resulting in a significantly higher Glu/GABA ratio （ P<0.01 ）. In comparison withthemodel group，ratsinboth theGYPXFgroupanddiazepam groupdemonstratedclinicalimprovementscharacterized by enhanced mental status and restored fur luster，along with significantly increased body weight（ P<0.05 ）. Specifically， rats in the GYPXF group exhibited significantly elevated GABA content（ P<0.05 ），significantly reduced Glu levels in hypothala1 nus（P<0.01） ），and significantly decreased Glu/GABA ratio（ P<0.01 ）. Rats in the Diazepam group showed more pronounced neurochemical alterations with significantly increased GABA content （ P<0.01 ）.Conclusion：GYPXF effectively ameliorates sleepdisordersinatswithliverdepresion-spleendeficiencyisomniaotentiallythroughadualmodulatioofneurotraier balance-upregulatingGABAsynthesiswhiledownregulatingGluproduction，therebyrectifying thepathologicalGlu/GABAimbalance in hypothalamic regulatory circuits.

KeywordsGanyu Pixu Formula； Insomnia; γ -aminobutyric acid；Glutamate中图分类号：R285.5;R338.63 文献标识码：A doi：10.3969/j.issn.2095-7130.2025.07.001

失眠是指尽管有合适的睡眠机会和睡眠环境，患者依然对睡眠时间和（或）质量不满足并影响日间社会功能的主观体验。（剩余6787字）