肉制品中牛源性成分的微滴式数字PCR定量方法研究

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Study on the Quantitative Method of Droplet Digital PCR for Bovine-DerivedMaterials in MeatProducts

HUANG Lin， HUANG Yang （Wuzhou Institute ofFood andDrugControl，Wuzhou543ooo， China）

Abstract： Objective： To address the problem of meat adulteration， a highly sensitive quantitative detection method for bovine-derived materials was established to distinguish between unintentional contamination and intentional adulteration，and to provide technical support for food safetysupervision.Method： Fresh beef was tested by droplet digital PCR using bovine-specific primers and probes，and a conversion model between beef quality and nucleicacid copy number was established.The specificity，accuracyandanti-interferenceabilityofthe method were verified by testing negative samples （chicken，mutton，pork），mixed meat samples and commercial bef products. At the same time，the feasibility of directly establishing a linear regression equation between beef quality and nucleic acid copy number for quantitative detection of bovine-derived materials was explored. Result： There was a significant linear relationship between beef quality and DNA content （ R2=0.998 7 ） and between DNA content and copy number （ R2=0.9968 ）. Based on this， the conversion formula between beef quality and copy number （ν= 0.037 6x-0.516 5 ）was derived，which can be used for the quantitative detection of bovine-derived materials.The method has good specificity，accuracy and strong anti-interference ability. The linear equation of beef quality and copy number （y=35.841x-11.523， R2=0.997 3 ） was directly established， which is suitable for quantitative detection of bovine-derived materials in meat products，simplifes the detection process，and improves the detection eficiency. Conclusion： Droplet digital PCR technology can accurately quantify bovine-derived materials in meat products with high accuracyand stronganti-interference ability，and is suitable for food adulteration identification and daily monitoring work of regulatory authorities.

Keywords： droplet digital PCR; quantitative detection; bovine-derived materials

食品掺假是指非法向食品中添加物理性状相似的物质，以降低成本、牟取利润或规避监管。（剩余5876字）