桔梗多糖的大孔树脂纯化工艺及其生物活性研究

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Study on Purification Process of Platycodon grandiflorum Polysaccharide by Macroporous Resin and Its Biological Activity

ZHANG Ziyi¹， CHENG Guo1， YANG Yi'， CHEN Jiayi'， YANG Yongjin12*

1.College ofLife Science and Technology，Hubei Engineering University， Xiaogan 43200， China; 2.Hubei Key Laboratory of Resource Utilization and Quality Control of Characteristic Crops， Xiaogan 4320o0， China）

Abstract： In this study，Platycodon grandiflorum was used as theraw material to extract crude polysaccharides from Platycodon grandiflorum by the water extraction and alcohol precipitation method. By comparing the purification effects of four diferent types of macroporous resins，namely AB-8，NKA-9，D1o1，and D3520，on Platycodon grandiflorum polysaccharides，the best resin was screened.The results show that the best resin screened outisAB-8，with a decolorizationrate of 93.82% ，aprotein removal rate of 90.74% ，andapolysaccharideretention rate of 79.88% .The Oxford Cup method antibacterial experiment indicated that both the polysaccharides of Platycodon grandiflorum before and afterpurification hadcertainantibacterial efectson Pseudomonas aeruginosa andEscherichia coli，andthedifference inantibacterial abilitybetweenthe two was relativelysmall.Theantioxidant activity of Platycodon grandiflorum polysaccharides was determined by measuring the hydroxyl radical scavenging rate.The results showed that at the same concentration，the purified Platycodon grandiflorum polysaccharides had stronger antioxidant activity.The research results can provide useful references for the development andutilizationof Platycodon grandiflorum polysaccharides.

Keywords： Platycodon grandiflorum polysaccharide; macroporous resin; purification; antioxidation; antibacterial

桔梗是桔梗科桔梗属的多年生草本植物，具有重要的药用和食用价值，在我国的东北、华北、四川、陕西等地区分布广泛，资源储量丰富。（剩余7238字）