高效液相色谱内标法测定益生菌类保健食品中低聚木糖的含量分析

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Analysis of Xylooligosaccharide Content in Probiotic Health Food by High Performance Liquid Chromatography Internal Standard Method

ZENG Lingcong （Guangzhou Inspection and Testing Certification Group Co.，Ltd.， Guangzhou 51140o， China）

Abstract： Objective： To establish a high performance liquid chromatography internal standard method for the determination of xylooligosaccharide content in probiotic health foods.Method： By optimizing the mobile phase to acetonitrile- 0.02mol⋅L-1 potassium dihydrogen phosphate solution （volume ratio at 30：70） ，inositol was selected as the internal standard，and the samples were derivatized.Result：The linearitywas good within theconcentration range of 0.05～2.50mgmL-1 1 （r2=0.9992 ）， the detection limit was 0.01mg⋅mL-1 ， the quantification limit was 0.03mg⋅mL-1 ，thespiked recovery rate was 94.1%～ 98.0% ，and therelative standard deviation was 0.45% ： Conclusion：This method is simple tooperate，accurate and reliable，andcan provide an effectiveanalytical means for the quality control of probiotic health food.

Keywords： high performance liquid chromatography intemal standard method; health food; xylooligosaccharides

低聚木糖作为功能性低聚糖，可调节肠道微生态，促进营养吸收[1-2]。（剩余3857字）