基于液相色谱-串联质谱法的农产品中氟苯尼考胺的检测方法优化

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Abstract： Objective： To establish a liquid chromatography-tandem mass spectrometry method for the detection of f lorfenicol amine in agricultural products， and to optimize the method. Method： 0.05% ammonia aqueous solution was used instead of traditional ammonium formate solution as the aqueous phase in the mobile phase， and the samples were extracted by 2% ammoniated ethyl acetate， defatted by n-hexane， ammoniated ethyl acetate reverse extraction， and determined by liquid chromatography-tandem mass spectrometry. Result： The linear relationship of the f lorfenicol amine was good at 1～50ng⋅mL-1 R2>0.999 ）， the recovery rate was 94.3%～ 112.7% ， and the relative standard deviation （RSD） was 1.9%～ 6.6% . Conclusion： This method can effectively solve the problems of abnormal peak emergence and matrix interference of f lorfenicol amine with high accuracy， and is suitable for the daily detection of f lorfenicol amine in agricultural products.

Keywords： f lorfenicol amine; liquid chromatography-tandem mass spectrometry; optimization of mobile phase

氟苯尼考胺（Florfenicol Amine，FFA）是氟苯尼考在动物体内的代谢产物，因与母体药物共同构成兽药残留风险，被列为联合检测指标 [1]。（剩余4297字）