基于PCR技术快速检测食品中的常见致病菌

打开文本图片集

Abstract： Objective： To establish a method based on polymerase chain reaction （PCR） to quickly and accurately detect common pathogens in food. Method： Escherichia coli O157：H7， Staphylococcus aureus and Listeria monocytogenes were selected as target pathogens， and primers were designed for their specific genes. The simulated contaminated food samples were tested and compared with the traditional culture detection method. Result： The PCR method can specifically amplify the target pathogen genes with a sensitivity of up to 102CFU⋅mL-1 ， and the detection time is shortened from 3 to 5 days of the traditional method to 6 to 8 hours. Conclusion： In actual food testing， the PCR method has a higher compliance rate than the traditional method， which provides effective technical support for the rapid detection of food microbial safety.

Keywords： PCR technology; food microbiology; pathogen detection; quick detection

食品安全问题一直是全球关注的焦点，尤其是由微生物污染导致的食源性疾病频发，严重威胁人类健康 [1-2]。（剩余3916字）