高效液相色谱-串联质谱法测定植物源食品中多抗霉素B

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Abstract： Objective： To establish a method for the detection of polyoxin B in plant-based foods by high performance liquid chromatography-tem massspectrometry. Method： The acetonitrile-water solution （the volume ratio of acetonitrile to water was 80：20 ） was added to the test sample， the extraction was carried out by shaking. The extracted solution was purified by an HLB solid-phase extraction column quantitatively analyzed using the external stard method.Result： The linear relationship of polymyxin B was good within the mas concentration range of 0.5μg⋅L-1 to 100.0μg⋅L-1 ， the correlation coefficient was greater than O.999.At the three addition level of 0.05， 0.10， 0.50mg.kg-1 ，theaverage recovery rate of polymyxinBinapples，tomatoes， cucumbers， spinach， rice， wheat was 83.6% to 101.3% ， the relative stard deviation was 2.3% to 7.8% ， the method quantification limit was 0.05mg⋅kg-1 . Conclusion： This method is simple to operate， highly sensitive accurate， is suitable for the determination of polymyxin B in plant-based foods.

Keywords： high performance liquid chromatography-tem masspectrometry; plant-based food; polymycin B

多抗霉素B是一种广谱性肽嘧啶核苷酸类农用抗生素，由可可链霉菌阿苏变种发酵产生。（剩余5195字）