液相色谱-串联质谱法检测畜禽产品中氟喹诺酮类药物残留量的方法优化研究

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Abstract： This study established a detection method based on Liquid Chromatography-Tandem Mass Spectrometry （LC-MS/MS） for the analysis of fluoroquinolone residues in livestock and poultry products.Based onNational Food SafetyStandard DeterminationofTetracyclines，Sulfonamidesand QuinolonesResidues in Animal Derived Foods byLiquid Chromatography-TandemMassSpectrometry （GB31658.17—2021），byoptimizing the pretreatment process （ 0.5% formic acid-acetonitrile was primarily used for extraction， followed by n-hexane degreasing and purification），the method wasvalidated in terms of linear range，spiked recovery，and precision.The validation results were as follows： the linear range of danofloxacin and norfloxacin in chicken and pork matrices was 2ng⋅mL-1 to 500ng⋅mL-1 （R2>0.99） ）， the limit of quantification could reach to 10μg⋅kg-1 ，the spiked recovery rate was 73.6% （204号 to 99.4% ，and the relative standard deviation wasless than 15% .The data indicate that the optimized method meets the experimental requirements.

Keywords： Liquid Chromatography - Tandem Mass Spectrometry （LC-MS/MS）; livestock and poultry products danofloxacin; norfloxacin; veterinary drug residues

氟喹诺酮类药物（Fluoroquinolones，FQs）因具有抗菌活性、良好的生物利用度和低毒性，已成为畜牧养殖业中防治细菌性感染的首选药物之一。（剩余3028字）