高效液相色谱法测定保健品中的芦荟苷含量

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Abstract： Objective： To establish a method for the determination of aloin content in health products using high performance liquid chromatography. Method： Samples were ultrasonically extracted with 55% methanol aqueous solutionby volume.Methanol- 0.1% phosphoric acid by volume was used as the mobile phase，and separation was performed on a CNW C18 column （ （250mm×4.6m m， 5.0μm ）under isocratic elution. Quantification was conducted using a standard calibration curve. Result： A good linear relationship was observed for aloin concentrations ranging from 0mg⋅L-1 to 50mg.L-1 ，with a correlation coefficient （R2） of 0.999 7. The method detection limit was 3mg⋅kg-1 to 15mg⋅kg-1 ，and the quantitation limit was 10mg⋅kg-1 to 50mg⋅kg-1 .For both solid and liquid samples，spiked recovery experiments were performed at 1，2，and 5 times the respectivequantitation limits，yielding recoveriesof 87.8%～99.7% and relative standard deviations of 2.11%～5.08% .Conclusion： The established method offers high recovery and good stability， making it suitable for qualitative and quantitative analysis of aloin in various types of health products.

Keywords： high performance liquid chromatography; health products; aloin

芦荟昔作为芦荟属植物的特征性成分，是由蒽醌类化合物与糖基结合形成的苷类物质。（剩余4981字）