多重荧光PCR在食品耐药菌检测中的应用

打开文本图片集

Abstract： Objective： In view of the problems that drug-resistant bacteria contamination in food threatens public health security and the limitations of traditional detection methods such as long detection cycle and low effciency，a multiplex fluorescence Polymerase Chain Reaction （PCR）technology detection system was established to simultaneously detect multiple drug-resistant genes， inorder to improve the detection efficiency.Method： Taking major food-bome drug-resistant bacteria such as Staphylococcus aureus and Escherichia coli as the research objects， specific primer probes were designed and a multiplex fluorescence PCR detection system was established. After optimizing the reaction system，detection and verification were carried out on samples such as meat products，dairy products and aquatic products. Result： The detection limit of the method reached 102CFU⋅mL-1 ， with good specificity. The detection time was shortened to 4h to 6h ，indicating that this method has good practical applicability and an accuracy rate of more than 95% .Conclusion： This technology can overcome the shortcomings of traditional methods， such as long time consumption and low throughput，providing a rapid and accurate detection means for food safety supervision.

Keywords： Polymerase Chain Reaction （PCR）; antibiotic-resistant bacteria; rapid detection; antibiotic resistance genes; food safety

抗生素滥用导致食品中耐药菌污染情况日益严重，对人类健康构成重大威胁。（剩余4711字）