高效液相色谱法测定果汁中的阿斯巴甜

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Abstract： To establish an eficient and accurate HPLC method for the quantitative analysis of aspartame in fruit juice， Agilent ZORBAX SB- ⋅C18 column （250mm×4.6mm，5μm） was used to isocratically elute acetonitrile and 0.1% phosphoric acid aqueous solution as the mobile phase， the flow rate was 1.0mL⋅min-1 ，the column temperature was 30∘C ，the detection wavelength was 210nm ，the injection volume was 10μL， ，and the sample was filtered by 0.45μm filter membrane （high-pulp samples need to be centrifuged first）and then injected directly. The results showed that the mass concentration of the target substance had a good linear relationship at 0.5μg⋅mL-1 to 100.0μg⋅mL-1 ， the correlation coefficient was more than 0.999， the limit of detection was 0.12μg⋅mL-1 ， the limit of quantification was 0.4μg⋅mL-1. ，and the average recovery was 98.5% to 101.2% .This method is accurate and reliable， suitable for thedailydetectionofaspartame in fruit juice drinks，andcan provideareliable analytical method for the quality control of fruit juice products.

Keywords： high performance liquid chromatography; aspartame; fruit juice; food safety

阿斯巴甜是一种高甜度（约为蔗糖的200倍）、低热量的人工甜味剂，因其协同增甜效果被广泛应用于无糖饮料、果汁等食品中[1-2]。（剩余4744字）