高效液相色谱-串联质谱法测定灵芝中3种麻黄碱含量

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Abstract： An analytic method was developedto investigate ephedrine hydrochloride，pseudoephedrine hydrochloride and methyl ephedrine hydrochloride in Ganoderma lucidum by high performance liquid chromatography-tandem massspectrometry.The analyteswere separated by gradient elution using 0.1% （v/v） ）formic acid aqueoussolution and 1 0.1% formic acid methanol solution as mobile phases.The analytes were extracted with 0.1% （v/v） formic acid aqueous solution and -0.1% （v/v） formic acid methanol solution （95：5）. The external standardization method was used forthe quantitative analysis.Thecalibration curves of the 3 target analytes were linearin the massconcentration range of 0～10ng/mL ，with correlation coefficients greater than 0.999.The method detection limit range and the method quantification limit range were 2.0～6.0μg/kg and 6.4～19.6μg/kg ，respectively.The average spiked recoveries for the 3 target analytes at three levels ranged from 97.3% to 112.0% ，and the relative standard deviations（RSDs） were less than 10% .The method is simple with highly sensitive，and can be used for theanalysis anddetermination of ephedrine hydrochloride，pseudoephedrine hydrochloride and methyl ephedrine hydrochloride in Ganoderma lucidum.

Keywords： high performance liquid chromatography-tandem mass spectrometry; ephedrine hydrochloride; pseudoephedrine hydrochloride; methyl ephedrine hydrochloride; Ganoderma lucidum

0引言

灵芝，属于药食同源的物质，能够补中益气，宁心安神，化痰正咳平喘，治疗咳喘、失眠、虚劳、不思饮食、心悸等。（剩余4681字）