UPLC-MS/MS法测定莓茶中安赛蜜等3种甜味剂

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Determination of Three Sweeteners Including Acesulfame in Ampelopsis grossedentata TeabyUPLC-MS/MS

OUYANG Weiqi， RAO Qingqing， ZHANG Hui （Xiangxi Prefecture Food and Drug Inspection Institute， Jishou 416ooo， China）

Abstract： In this paper， an ultra-performance liquid chromatography tandem mass spectrometer was established for the determination of acesulfame potassium，cyclamate and alitame in Ampelopsis grossedentata tea. The Ampelopsis grossedentata tea samples were extracted by pure water vortex ultrasonication，and then centrifuged with precipitant precipitation. With 5 mmol L-1 ammonium acetate water- 0.1% forme methanol was used as mobile phase，gradient elution was used，Atlantis T3columnseparation，external standard qantification，and multi-reaction monitoring negative ion mode scanning.The results showed thatthe three sweeteners had a good linear relationship within their respective linear ranges （ （R2>0.999） ， and the limit of quantification was 0.003mg.kg-1 to 0.007mg.kg-1 The recovery rate of spike under the three levels was 83.02% to 99.52% ，and the relative standard deviation was less than or equal to 8.02% ，which met the detection requirements. This detection method is simple，reproducible， sensitive，and environmentally friendly，and can be used for the detection and daily monitoring of acesulfame potassium， cyclamate and alitame in Ampelopsis grossedentata tea.

Keywords： ultra-high performance liquid chromatography-tandem mass spectrometry; Ampelopsis grossedentata tea; acesulfame potassium; sodium cyclamate; alitame

莓茶，葡萄科蛇葡萄属显齿蛇葡萄（Ampelopsisgrossedentata）植物，广泛分布于云南、湖南、湖北、广东、广西、贵州等地，全国种植面积超1.3万 hm2 莓茶史籍记载和食用历史悠久，富含黄酮和多糖类活性成分，具有降血糖、保肝护肝等健康功效。（剩余5744字）