高效液相色谱法同时测定食品中8种非留体抗炎药

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Simultaneous Determination of Eight Non-Steroidal AntiInflammatory Drugs in Food by High Performance Liquid Chromatography

LUO Haiyan， HE Xi* ，YANG Fang， HAO Rui， YANG Fan， GU Jinhong （Bayinguoleng Mongolian Autonomous Prefecture Institute for Food and Drug Control， Korla 8410oo，China）

Abstract： A high-performance liquid chromatography method was established to determine the content of 8 kinds of non-steroidal anti-inflammatory drugs illegally added to food. 1g of the sample was weighed， 10mL of 1% glacial acetic acid methanol solution was added for ultrasonic extraction， separated by C18 column （250mm× 4.6mm ， 5μm ），and thephosphoric acid aqueous solution with pH=2.5 wasused as mobile phaseAand methanol as mobile phase B for gradient elution，and the detection wavelength was 220nm ，and the external standard method was used for quantitative quantification.The results showed that the 8 NSAIDs had a good linear relationship with their corresponding chromatographic peak areas in the range of 0.5μg⋅mL-1 to 20.0μg⋅mL-1 ，and the correlation coefcients were all above 0.999.The method has good acuracy and precision，and is suitable forthe detection of non-steroidal anti-inflammatorydrugsinfood.

Keywords： high-performance liquid chromatography; non-steroidal anti-inflammatory drugs; food

非甾体抗炎药（Non-Steroidal Anti-InflammatoryDrugs，NSAIDs）是一类非类固醇激素类药物，临床上被广泛用于多种免疫功能紊乱炎性疾病及各种疼痛症状的缓解，副作用较为明显，主要会累及胃肠及皮肤附件系统[1-4]。（剩余3854字）