复合酶辅助提取菊花总黄酮工艺研究

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Study on Complex Enzymatic-Assisted Extraction of Total Flavonoids of Chrysanthemum morifolium Ramat.

ZENG Xiaotong1²，LI Yusi1³，LI Wenyi'， WEI Wei’， ZHANG Xin1， HU Qin' （1.College ofChemistryand Chemical Engineering，Sichuan UniversityofArtsand Science，Dazhou 635ooo，China; 2.Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province， Dazhou ， China; 3.Bioengineering College， Chongqing University， Chongqing 400040， China）

Abstract： The total flavonoids of Chrysanthemum morifolium Ramat. were extracted using a composite enzyme-assisted method，with the total flavonoid extraction amount as the evaluation index.Single factor and orthogonal experiments were conducted to optimize the composite enzyme-asisted extraction process.The results showed that the optimal extraction conditions for the total flavonoids of Chrysanthemum morifolium Ramat. were a composite enzyme amount of 50mg ，an extraction time of 80 minutes， and a composite enzyme ratio of 1：1 Under these extraction conditions， the total flavonoid extraction amount was 38.75mg⋅g-1 . The composite enzyme-assisted extraction method is eficient and gentle，which can provide a reference for the development and application of the total flavonoids of Chrysanthemum morifolium Ramat..

Keywords： Chrysanthemum morifolium Ramat.; total flavonoids; complex enzyme; orthogonal test

菊花为菊科植物菊（ChrysanthemummorifoliumRamat.）的干燥头状花序，是中国传统十大名花之一，是著名的药食同源植物，具有清热解毒、平肝明目等作用[1-2]，广受当代大众追捧。（剩余3649字）