HPLC-MS/MS测定白菜中氟唑菌酰胺的不确定度评定

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Uncertainty Evaluation for Determination of Fluxapyroxad in Cabbage by HPLC-MS/MS

WU Yu'e'， WANG Qing²， HUANG Yongqiao3*

（1.Guiyang Agricultural Products Logistics Development Co.，Ltd.， Guiyang 55oo0， China; 2.QianxinanPrefectre

Agriculure and Rural Affairs Bureau， Guizhou Provine，Qianxinan 562400， China; 3.Guizhou Testing Technology Research and Application Center， Guiyang 55oo14， China）

Abstract：A mathematical model was established based on the standard method of GB 2320o.121—2021 to evaluate the uncertainty of the high performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS） method for determining the content of fluconazole in cabbage. Based on the system identification and quantitative analysis of the contributing factors of uncertainty in each step of the detection process，the composite standard uncertainty is calculated. The results showed that the content of fluxapyroxad in cabbage was 0.028mg-kg-1 ，with an extended uncertainty of 0.008mg⋅kg-1 （k=2） .The preparationof standard solutionand fitting of standard curve were the main uncertainties introduced during the measurement process.

Keywords： high performance liquid chromatography-tandem mass spectrometry; cabbage; fluxapyroxad; uncertainty

氟唑菌酰胺（Fluxapyroxad）是由巴斯夫公司研发的一种羧酸酯类化合物，其作用机制主要基于抑制病原菌呼吸作用，从而阻断其代谢过程以达到控制病害作用[1-2]。（剩余6483字）