油菜花粉中3种化学成分含量的测定

打开文本图片集

Determination of Three Chemical Components in Rapeseed Pollen

HUANG Xiaoqiang，LI Luyao， WANG Sijie， CHANG Tianyi， YAN Chunsheng， JIA An （Medical School，Huanghe Science & Technology College， Zhengzhou 45oo63， China）

Abstract： Objective： To establish a high performance liquid chromatography （HPLC） method for the simultaneous determination of rutin，quercetin and kasanferol contents inrapeseed polln.Method： An Agilent Poroshell 120EC-C18 chromatographic column was used， with a gradient elution using methanol- .0.4% phosphoric acid as the mobile phase. The flow rate was 1mL⋅min-1 ，the injection volume was 5μL. ，and the column temperature was 30∘C . The detection wavelength is 368nm .Result： The correlation coefficients of rutin， quercetin， and kaempferol were all greater than 0.999 within their respective concentration ranges，indicating a good linear relationship.The relative standard deviations （RSD） of the three substances in the precision experiment were 0.62% 0.65% ，and 0.51% 0 respectively. In the stability experiment， the RSD of the three substances were 3.26% 0 2.70% and 1.86% ，respectively. In the repeatability experiment， the RSD values of the three substances were 1.29% 1.85% ，and 2.85% ，respectively. The average recovery rates were 100.44% 99.34% ，and 99.65% ，respectively. Conclusion： This determination method is easy tooperate，has high precision，good stability，accurateandreproducible detection results，andcanbeused for the determination of rutin， quercetin and kaempferol content in rapeseed pollen.

Keywords： rapeseed pollen; high performance liquid chromatography （HPLC）; rutin; quercetin; kaempferol

油菜（Brassicarapavar.oleiferaDC.）属十字花科芸臺属，学名芸耋[。（剩余4143字）