SPE-HPLC法测定食品中对羟基苯甲酸酯类防腐剂

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Determination of p -Hydroxybenzoate Preservatives in Foods by SPE-HPLC

GUO Fuchao， WANG Tianping，HUANG Hecheng， SUN Yayun， CAO Ailing （Yunnan Yunce Quality Testing Co.， Ltd.， Kunming 650501， China）

Abstract： A analytical method for the determination of seven kinds of p-hydroxybenzoate preservatives in foods by solid phase extraction combined with high performance liquid chromatography is established.The sample pretreatment process and detection method are improved by optimizing the solid phase extraction conditions and the separation parameters of high performance liquid chromatography. The results show that this method has good separation efect and high sensitivity for the seven common p-hydroxybenzoates in foods，the linear range is good within the range of 0.2mg-L-1 to 50mg⋅L-1 （the correlation coefficients are all greater than O.999）， the average recovery rate is from 82.4% to 95.8% ，therelative standard deviation is from 0.35% to 3.18% ，and the limit of detection is from 0.18mg.L-1 to 0.27mg.kg-1 ， which can meet the accurate detection requirements of p-hydroxybenzoate preservatives in foods.

Keywords： solid phase extraction; high performance liquid chromatography; food; p-hydroxybenzoate

对羟基苯甲酸酯类防腐剂，凭借广谱抗菌特性、高效抑菌能力及低毒性优势，在食品与化妆品领域应用广泛。（剩余4795字）