野生大麦EIL基因家族成员鉴定与表达分析

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关键词：野生大麦（Hordeumspontaneum）；EIL基因家族成员;鉴定;表达分析

中图分类号：S512 文献标识码：A

文章编号：0439-8114（2025）08-0226-04

DOI：10.14088/j.cnki.issn0439-8114.2025.08.034 开放科学（资源服务）标识码（OSID）：

Identification and expression analysis of members in the EIL gene family of wild barley

HU Huila，MENRui-long1bed， ZHAO Ming-zhuo1bed，SUN Ming-ruo1bed，XU Jun-ying1bed， LIUHai-yang 16cd ，YANG Long-weibcd，TIAN Yu²，YUWei-pu³，QIU Xian-jin1bed （aColegeftsdecggseactofdEoulUlatiostfucaK LaboratoryofGrnandEficientProuctioofCopsinteMdleReachesofteYangtzeRiver，MinistryofAgricultureadRuraairs constructedbdoce）;dle，gtUvesityg4ubeina;2a AgriculturalSciene，Yichang44304，Hubei，hina;3.JingzhouAcadeyofAgriculturalSciences，Jingzhou340，Hubeihina）

Abstract：MembersoftheEILgenefamilyinwildbarley（Hordeumspontaneum）wereidentifiedbasedonwhole-genomeanalysis technology；thecompositionanddistributioncharacteristicsofcis-actingelementsintheirpromoterregionswereanalyzed；andtheir expresionpatersunderstressconditionswere investigated tomine potential stress-resistantregulatorygenes.Theresultsshowed thattheEIL genefamilyinwildbarleycontainedsevenmembers，namelyHEI1，HEI2，HEIL3，HEIL，HEIL5，HEIL6，and HuEIL7.Thesevenmembershadanaverage proteinlengthof489aa，ameanmolecular weightof53790.52u，isoelectricpontsrangingfrom489to6.7，andistabilityindicesetween4.and.39;allwerehydrophilicproteisandlocalizedintenucleus.Cis actingelementsinthepromoterregionsofmembersintheEILgenefamilyofwildbarleytotaled14types，mainlyincludinglightresponsiveelements，planthormone-esponsiveelements，andstre-responsiveelements.Inranscriptomedataanalysisofwildbarley，geneexpresiolevelsvarieduderdiffrenttreamentsHEIL，HEIL，andHEI7showednosignificantdieensinexpresionlevelsundercontroltreatment，droughtstresstreatment，orrehydrationtreatmentafterdrought，indicatingthattheegenes mightnotparticipateindroughtstressesponse.TesuppressionofHEIL4expresionasassociatedwithehanceddroughtresistance，suggesting that it might play a negative regulatory role in barley drought tolerance.

KeyWords：wildbarley（Hordeum spontaneum）；membersoftheEILgenefamily；identification;expressionanalysis

干旱是影响作物生长和产量的主要限制因素，在干旱条件下，田间作物产量损失可达 30%～ 90%[1] 。（剩余5921字）