基于相对迁移率的2个山羊草二倍体物种HMW-GS组成分析

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关键词：顶芒山羊草（Ae.comosa Sm.，MM， 2n=2x=14 ）；尾状山羊草[Ae.markgrafi（Greuter）K.Hammer， CC， 2n=2x=14 ;HMW-GS;遗传多样性；聚类分析

中图分类号：S545 文献标识码：A文章编号：0439-8114（2025）08-0178-05DOI：10.14088/j.cnki.issn0439-8114.2025.08.027

Analysis of HMW-GS composition of two Aegilops diploid species based on relative mobility

ZHANG Hai-hui¹，ZHANG Yan-ru1，SONG Lin-Xuan2，HAO Zhi-hua²， ZHANG Yi-lin1，WEI Sa²，HU Xi-gui² （1.Schoolof Biological Engineering，Xinxiang Instituteof Engineering，Xinxiang 4537oo，Henan，China; 2.School ofAgriculture，Henan Institute ofScience and Technology，Xinxiang 453oO3，Henan，China）

Abstract：Aegilopsisconsideredasanimportantgenesourceforimprovingmodercommonheat，whichhasmanyexcelentgenesor traits.Inrdertounderstandthegeneticdiversityofhighmolecularweightgluteninsubunits（HMW-GS）inAe.diploidspecies，odiumdodecylsulfate polyacrylamide gelelectrophoresis（SDS-PAGE）was usedtoanalyze HMW-GS in40Ae.acession（Ae.comosa andAe.markgrafi）.Theresultsshowedthetotalof17elativemobilitieswereidentifiedamong the40acessions，ofichfiveelativemobilities（0.82～0.98）werelowerthanthatofChineseSpring1Dx2（1.0O），andonerelativemobilty（1.49）washigherhanthat of ChineseSpring1Dy12（1.40）.Analysisof geneticsimilaritycoeficients（GS）among theaccessionsrevealedarangeof0.00～00， withanaveragevueofO3.And，itasfoundtatteverageG（.58）ofAe.markgraficessosassiificantlygran that of Ae. comosa accessions（O.31）. The clustering results showed that 40Ae . accessions were divided into two groups at the GS 0.10， of which19Ae.comosaacessonswerecusteredinGroup1.And，theGroup2inluding21Ae.ccessonscouldbefurtherdividedinto two sub-groups，which indicated that Ae.accessions clustering together had the same species.

KeyWords：Aegilopscomosa;Ae.markgrafi； HMW-GS；genetic diversity;clustering analysis

山羊草属（AegilopsL.）是禾本科（Pooideae）小麦族（Triticeae）中一个重要属，含有23个物种，其包括11个二倍体物种、10个四倍体物种和2个六倍体物种[1]。（剩余8685字）