链霉菌JXJA01发酵培养基与发酵条件优化及其对南方根结线虫的抑制作用

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关键词：欧洲疮痂链霉菌（Streptomyces europaeiscabiei）JXJAOl;南方根结线虫（Meloidogyne incongnita）；发酵条件优化；生物防治

中图分类号：S476.1;Q939.96 文献标识码：A

文章编号：0439-8114（2025）08-0131-09

DOI：10.14088/j.cnki.issn0439-8114.2025.08.020 开放科学（资源服务）标识码（OSID）：口

Optimization of fermentation medium and conditions of Streptomyces sp.JAJA01 and its inhibition on Meloidogyne incognita

ZHANG Fan，FANLin-juan，WUCai-yun，YUANMeng-yu，YAO Jian，LIU Zi-rong，YAO Ying-juan，XUXue-liang （IsitutefAglualAppldcrobooJagiProvcialKyLaboratoofgicultualotSouePltioCotoldate ComprehensiveUtilization，Jiangxi Academy ofAgriculturalSciences，Nanchang 33O2Oo，China）

Abstract：TofurtherenhancetheinibitoryefectoftreptomyceseuropaeiscabieistrainJXJAOlonsecond-instarlarvaeofMeloido gyne incognita（southenrot-knotnematode），thecorrectedmortalityrateofthestrainagainstsecond-instarlarvaeofMelodogyne incognitawasusedasthescreeningindex.Theoptimalfermentationmediumwasfirstsreeedthroughsingle-factorexperiments，and onthis basis，responsesurfaceanalysis wasconductedtooptimizeandobtainthebestfermentation medium formulaandoptimalfermentationconditions.Theresultsshowed thattheoptimalfermentationmediumforthistrainwasfermentationmediumD.Furtheroptimization experiments on fermentation conditions revealed that when the fermentation medium contained 8.57g/L soluble starch，2.31 g/L urea， 0.34g/L ammonium sulfate， 0.40g/L calcium carbonate，and 0.40g/L sodium chloride，with a fermentation temperature of （204号 28qC ，an inoculum size of 6% ，an initial pHof 7.6，and afermentation time of 5 days，the corrected mortality rate of the strain’s fermentation broth against second-instar larvae of Meloidogyne incognita reached 96.78%±1.89% ，which was 22.73 percentage points higher than that before optimization.

KeyWords：StreptomyceseropeiscabieiJXJAO;Melodogeincognita;ptizatioofmenationcodis；ioloicalcotrol

根结线虫（Meloidogynespp.）是寄生性植物病原线虫，寄生于植物根系，其寄主范围广泛，能侵染3 000多种植物，对农业生产造成严重危害[1.2]。（剩余12921字）