基于代谢组学分析根瘤菌调节因子nolR对大豆根系代谢的影响

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关键词：大豆[Glycinemax（L.）Merr.]；根瘤菌；调节因子nolR；代谢组学中图分类号：S565 文献标识码：A文章编号：0439-8114（2025）08-0219-07DOI：10.14088/j.cnki.issn0439-8114.2025.08.033开放科学（资源服务）标识码（OSID）：

Metabolomicsanalysisoftheeffectsoftherhizobial regulatornolRon rootmetabolismin soybean

BI Yu a ， ZHU Jia-nana，ZHEN Taoa，TIAN Shuanga，WU Hao-qionga，YU De-shuiaʰ，GAO Dong-yuan' （a.InstituteofMicrobiology；b.OfficeofthePartyCommitee;c.InstituteofPetrochemistry，HeilongjiangAcademyofSciences， Harbin 150040，China）

Abstract：Metabolomicstechnologywasemployed toanalyzediferences inootmetabolitesofsoybeanGlyeinemax（L.）Mer.ultivarHeike6OafterinoculationwithSinorhizobiumfredi HH1O3（hereinafterreferrdtoasrhizobium HH103）anditsmutant HH103△nolR.Theresultsshowedthatatotalof99O7diferentialmetaboliteswereidentifiedbetweesamplesfromtherhizobium HH103treatmentandthe mutant HH103△nolR treatment，among whichthecontentof 4202 metabolites was upregulatedand thatof 5705metabolites wasdownregulated.Lipidsandlipid-likemolecules，asellasphenylpropanoidsandpolyketides，accountedfora relativelyhighproportionamongthdiferentialetabolitesMalvinebitedtelargesticreaseidierentialmetaboliteontentfterinoculationwiththeizobium，whilePhysalin_Fshowedthelargestdecrease.Tediferentialmetabolitesweresignificantlyenrichedinpathwayssuchasgalactosemetabolism，ysinebiosynthesis，porphyrinmetaboism，soqunolinealkaloidbiosythsis，and propanoate metabolism.Among ediferentialmetabolites，thecontentdiferencsinfavooidompoudswereelativelysigficant， and the deletion of the nolR gene promoted the metabolism of flavonoid compounds in soybean.

Keywords：soybean[Glycine max（L.）Merr.]；rhizobium；regulator nolR；metabolomics

大豆[Glycinemax（L.）Merr.]是中国重要的粮食作物，黑龙江省作为中国优质大豆的主产区，需要探索高效且可持续的农业生产方式来提高大豆的产量与质量。（剩余8950字）