金耳T-1的生物学特性及全基因组测序分析

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中图分类号：S646.6 文献标识码：A

文章编号：0439-8114（2025）08-0152-08

DOI：10.14088/j.cnki.issn0439-8114.2025.08.023 开放科学（资源服务）标识码（OSID）：

Biological characteristics and whole genome sequencing analysis of Naematelia aurantialba T-1

TIAN Shuang，BIYu，GUO Yan，WU Hao-qiong，YE Yang，LIU Jia-ning，ZHU Jia-nan （Institute of Microbiology，Heilongjiang Academy of Sciences，Harbin 15Oo1O，China）

Abstract：ThesporesofNaemateliaurantialbaT-1wereusedastheexperimentalmaterial.ThebiologicalcharacteristicsofNematelia urantialbawere studied，anditswholegenomewassequencedandanalyzed.Theresultsindicatedthatheoptimalcarbon sourceforT-wassucrose，theoptimalnitrogensourcewaspeptone，theoptimalinorganicsaltwaspotassumchloride，theostsuit able pH was 7，and the optimal inoculation amount was 108 cells/mL. The sequencing quality of the genomic bases was between 35 and 40，which wasrelativelygood.The ATandGCbasecontents wererelativelyuniform，andthesequencingresultswerenormal. ThroughK-merfrequencyanalysis，thesizeof heT-genomewasdeterminedtobe26612159bp.After thesequencingdatawere assembled，it was shown that the N5O value was relatively high，and the GC content was 56.45% . The display of the genome using Circos software indicated that the full-length of the T-1 genome was 18 180 385 base pairs，with a GCcontent of 56.46% . The genome wasdividedinto1Ocontigs.ThephylogenetictreeshowedthatT-1wasmostcloselyclusteredwithNaemateliaurantialbastrans. TheGOfunctionclasificationwasannotatedintothre majorcategories，mongwhichtegnesivolvedinbiologicalprocssswere themost.AftercomparisonwiththeKEGGdatabase，atotalof4O96genes wereannotatedintofivemajor metabolicpathways，among which the genes involved in metabolic pathways were the most.

Key Words：Naemateliaaurantialba；biological characteristics；whole genome sequencing；functional prediction;pathwayenrich mentanalysis

金耳（Naemateliaaurantialba）是一种可食用真菌，形状呈脑状，表面光滑，颜色在转色前呈白色，转色后呈金黄色[12]。（剩余10378字）