勋西马头山羊线粒体DNAD-loop区遗传多样性分析

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关键词：郧西马头山羊（Caprahircus）;线粒体DNA；D-loop区；遗传多样性

中图分类号：S827 文献标识码：A

文章编号：0439-8114（2025）07-0198-05

DOI：10.14088/j.cnki.issn0439-8114.2025.07.034 开放科学（资源服务）标识码（OSID）：口

Genetic diversity analysis of mitochondrial DNA D-loop region in Yunxi Matou goat

CHENGLei，LIUChen-hui’，CHENYang1，XIANGMin1，YUJie1，ZHONGZhu-xia1， XIAYong-chun²，YANGWu-jun³，HUXiu-zhong1

（1.InstituteofAnimalHusbandryandVeterinaryMedicine，WuhanAcademyofAgriculturalSciences，Wuhan43O65，China; 2.Yunxi Qingqing Matou Goat Breeding Cooperative，Shiyan 442624，Hubei， China; 3.Yunxi County Animal Husbandryand Veterinary Service Center，Shiyan 442624，Hubei，China）

Abstract：ThegeneticdiversityofYunxiMatougoat（Caprahircus）wasevaluatedbasedongeneticvariationanalysisofmitochondrial DNA（mtDNA）D-loopregionsequences.Thecomplete mtDNA D-loop sequences of 184 Yunxi Matou goats were obtained by PCR amplificationandsequencing，folowedbysystematicanalysisof nucleotidepolymorphismandhaplotypediversity，neutralitytests （Tajima’s D and Fu’s Fs ），mismatch distribution analysis，and cluster analysis with mtDNA D-loop sequences from different goat breeds.The results showed that the proportions of A， G ，C，and Tbases in mtDNA D-loop of Yunxi Matou goat were 30.77% ， 14.26%， 26.26% ，and 28.71% respectively，with A+T content （ 59.48% ）significantly higher than G+C content （ 40.52% ）.There were 52 haplotypesand131variable sites inmtDNAD-loopofYunxiMatougoat，ncluding51singleton variablesitesand80parsimonyinformative sites.Fu's Fs test showed Fs value of -2.48 ，indicating significant deviation from the neutral model（ P<0.05 ）.The phylogenetictreeofYunxi Matou goatandotherfivegoatbreeds（Chuandong White goat，Shannan White goat，Huanghuai goat，Beichuan Whitegoat，andBoergoat）showedthat184individuals weredividedintothreemajorbranches，ncluding twonativebranchesand oneBoergoathybridbranch.TheYunxi Matougoatpopulationshowedhighpolymorphismandrichgeneticdiversitywithgreatbreeding potential，but exhibited genetic introgression from surrounding local goat breeds and Boer goat.

Key Words：Yunxi Matou goat（Capra hircus）；mitochondrial DNA;D-loop region； genetic diversity

马头山羊是中国优良地方肉用山羊（Caprahircus）品种，主要分布于湖北、湖南等地，具有性成熟早、繁殖性能高、耐高温高湿环境等特性[1]。（剩余7320字）