多根紫萍SpLAR基因的克隆、表达分析及表达载体构建

打开文本图片集

关键词：多根紫萍（Spirodela polyrrhiza）；SpLAR基因；生物信息学；表达分析；载体构建中图分类号：R932；Q812 文献标识码：A文章编号：0439-8114（2025）07-0120-08DOI：10.14088/j.cnki.issn0439-8114.2025.07.021

开放科学（资源服务）标识码（OSID）：

Cloning，expression analysis and expression vector construction of the SpLAR gene in Spirodela polyrrhiza

LIUWen-ying，ZUOYan-xi，HEShu-ping，YANGPu，XIANGBei-bei （SchoolofChinese Materia Medica，Tianjin UniversityofTraditional Chinese Medicine，Tianjin 301617，China）

Abstract：Toinvestigatetheroleoftheleucoanthocyanidinreductase-encodinggeneSpLARinproanthocyanidinbiosynthesisinSpiro delapolyrrhiza，thefull-length SpLAR cDNA sequence was obtained from transcriptome data and the gene was successfully cloned. The characteristics of the SpLAR protein were predicted，and the expression levels of the SpLAR gene under different culture days and nutrientstressconditionsweredetectedAditinall，bymeasuringtheaccumulatioofproanthocyanidnsudercoespondingoditions，the correlation between the expression level of the SpLAR gene and the accumulation of proanthocyanidins was further analyzed. ThepCAMBIA3Ol-SpLARplantexpresionvectorwasconstructedusinghomologousrecombinationtechnologyBioinformatisanalysisresultsshowedthatthegenewas1O59bpinlength，encoding352aminoacids，withapredictedmolecularweightof38ku.Itwas ahydrophilicandunstable protein，containingone transmembrane domain，having nosignal peptide，and belonging to the NADB_Rossmannsuperfamily.TheSpLARproteinhadthehighestsimilaritywiththehomologous proteinfromColocasiaesculenta. RT-qPCRandcontentdeterinationresultsindicatedthattheexpresionleveloftheSpLARgeneshowedadynamictrendofirstincreasingandthendecreasing，andwaspositivelycorelatedwiththeaccumulationofproanthocyanidins.UnderDatkotreatment，the expression level of the SpLAR gene was the highest at 9d，and at this time，the accumulation of proanthocyanidins reached a peak of 20.6mg/g .Inaddition，thenutrientstresstreatmentcouldsignificantlypromotetheexpressionofthe SpLARgeneandthebiosynthesis of proanthocyanidins，increasing the accumulation to 24.8mg/g at 9 d.

KeyWords：Spirodela polyrrhiza；SpLAR gene;bioinformatics；expressionanalysis；vector construction

浮萍（Duckweeds）隶属于天南星科浮萍亚科（Lemnoideae），该亚科包含5个属，分别为紫萍属（Spirodela）斑萍属（Landoltia）青萍属（Lemna）、扁无根萍属（Wolffiella）以及无根萍属（Wolffia）[]。（剩余13225字）