深秋红沙棘NHX1基因克隆与生物信息学分析

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关键词：深秋红;沙棘（Hippophaerhamnoides）;HrNHX1基因;基因克隆;生物信息学分析

中图分类号：S793.6 文献标识码：A

文章编号：0439-8114（2025）07-0192-06

DOI：10.14088/j.cnki.issn0439-8114.2025.07.033 开放科学（资源服务）标识码（OSID）：

Cloning and bioinformatic analysis of NHXl gene in Shenqiuhong Hippophae rhamnoides

CUI Chang-zhena，HUO Yan-boab，ZHANG Si-yua，CHAI Guai-qianga.b，BAO Liang-liangb，DUAN Yi-zhong.b （a.College of AdvancedAgricultural Sciences；b.Yulin Genuine QinMedicinal Materials Research Institute，Yulin University， Yulin 719000，Shaanxi，China）

Abstract：UsingShenqiuhongHippophaehamnoidesastheexperimentalmaterial，thegeneencodingtheNHX1proteiof heNa+/H+ antiporter（NHX）familywascloned，andbioinformaticanalysis was performedonthe HippophaerhamnoidesNHX1protein（HrNHX1）.Theresults indicatedthatthefullengthofthe HrNHX1 gene wasapproximately6OObp，encodinganacidic hydrophobicpro tein consisting of 538 amino acid residues.The molecular formula of this protein was C2754H4269N672O759S21 ，with a total of 8 502 atoms andarelativemolecular massof 59637.71.HrNHX1belonged toastableprotein，containing13transmembrane domains，ackinga signalpeptid，beingano-secretoryproteinndcontainingoneNa+/HexchangerdomaincharacteristicoftheNHXproteinfamily. Subcelularlocalizationanalysisshowedthatthe HNHX1proteinwaslocalizedonthevacuolarmembrane.Homologyalignmetanalysis indicated thattheHrNHX1proteinsequencehadhighsequencesimilaritywiththeNHX1proteinsequencesofGosypiumaimondi，Gossypiumhirsutum，Morusnotabilis，ndHeveabrasiliensis.PhylogenetictreeanalysisfoundthattheHrHX1proteinsequence clusteredonthesameevolutionarybranchwiththeNHXproteinsequencesofriceandwheat，whichhaddroughtresistanceandsalt tolerancefunctions，sugestingtheyhadacloseevolutionaryrelationship.The HrNHX1geneplayedanimportantroleinenhancing plantdroughtresistanceandsalttolerance，andregulatingtheactivityofitsencodedHrNHX1proteinorthexpresionlevelofthis gene could effectively improve plant adaptability to drought and salt stress.

Key words： shenqiuhong; Hippophae rhamnoides； HrNHXl gene;gene cloning；bioinformatic analysis

沙棘（Hippophaerhamnoides）属于胡颓子科（Elaeagnaceae）沙棘属（Hippophae），是一种具有显著经济价值和生态价值的植物[1]。（剩余7097字）