杨梅中5种杀菌剂多残留检测方法比对

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关键词：杨梅（MorellarubraLour.）；杀菌剂；农药残留；检测方法；免疫层析法；比对中图分类号：TS207 文献标识码：A文章编号：0439-8114（2025）07-0164-07DOI：10.14088/j.cnki.issn0439-8114.2025.07.028开放科学（资源服务）标识码（OSID）：[

Comparative study of five fungicides multi-residue detection methods in Morella rubra Lour.

JIA Hui-yan，FU Yan，WANG Quan-sheng，WU Yin-liang （Ningbo Academy of Agricultural Sciences，Ningbo 315O4O，Zhejiang，China）

Abstract：Using MorellrubraLour.astheresearchsubject，fivefungicides withhighdetectionrates（carbendazim，difenocoazole， pyraclostrobin，azoxystrobin，tebuconazole）were srenedbasedonhistorical pesticideresidues monitoringdatafromthe Testing Centerof NingboAcademyof Agricultural Sciences.Asystematiccomparisonwas madeonthetechnicalperformanceoftwodetection methods：collidal goldimmunochromatographicrapiddetectionandultra-performanceliquidchromatography-tandemmasspectrometry（UPLC-MS/MS）.Theresultsshowedthatthedetectionlimitsoftheimmunochromatographymethodforthefivefungicideswere （20 0.20mg/kg （carbendazim）， 1.00mg/kg （difenoconazole）， 5.00mg/kg （pyraclostrobin）， 5.00mg/kg （azoxystrobin），and 1.00mg/kg （204 （tebuconazole），respectively.The detection limits of the UPLC-MS/MS method forallfive fungicides were 0.001mg/kg .When the spiked levels of the five fungicides in Morella rubra Lour. were 0.010～1.000mg/kg ，the UPLC-MS/MS method demonstrated good accuracy and precision. The average recoveries of the five fungicides ranged from 74.8% to 107.5% ，with relative standard deviations （ RSD ）between 1.1% and 9.0% .The immunochromatography method exhibited good precision.The false-negative rate for the five fungicides was O，the false-positive rate was ⩽5% ，and the relative accuracy was ⩾97.5% .Although the detection limits of the immunochromatographymethodwere higher，theywereallbelowthe maximumresiduelimits.This methodwasfullyaplicableforqualitative screening of pesticide residues and met the timeliness and reliability requirements foron-site rapid detection.

KeyWords：MorellrubraLour.;fungicides；pesticideresidues；detectionmethods；immunochromatographycomparativestudy

杨梅（MorellarubraLour.是中国长江中下游地区重要的特色经济作物，果实风味独特，同时还有药用价值，内含多种抗氧化物和黄酮类化合物，具有消炎、抗过敏及对癌细胞的抑制等作用[12]。（剩余10134字）