平板计数法测定果酒中菌落总数的不确定度分析

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摘 要：为了提高实验室检测结果的准确度，本文结合食品微生物检验菌落总数测定的最新标准与评定测量不确定度的相关规定要求，分析果酒中菌落总数不确定度的来源，采用合并样本标准差的方法来评定菌落总数的不确定度。结果建立了果酒中菌落总数的不确定度评定方法，测量不确定的扩展不确定度为0.079 3。

关键词：菌落总数;不确定度;果酒

Uncertainty Analysis of Total Number of Colonies in Fruit Wine by Plate Counting Method

ZHANG Xiaoyue

（Wuliangye Group Co.， Ltd.， Yibin 644007， China）

Abstract： In order to improve the accuracy of laboratory test results， the latest standards for total bacterial count determination of food microbiological test and related requirements for evaluation of measurement uncertainty was combined in this paper， the source of total uncertainty of bacteria in fruit wine was analyzed and the method of uniting standard deviation was applied to determine the uncertainty of the colony. The results showed that the method was established to evaluate the uncertainty of total number of bacteria in fruit wine， and the extended uncertainty of measurement uncertainty was 0.0793.

Keywords： total number of colonies; uncertainty; fruitwine

随着实验室认可和资质认定工作的开展[1]，根据《General Requirements for the Competence of Testing and Calibration Laboratories》（IS0/IEC 17025：2017）中相关规定[2]，测量不确定度评定是检验检测实验室必备的能力之一，因此越来越多的实验室意识到测量不确定度评定的重要性[3]，在实验室的日常检测中，积极开展方法不确定度评定工作。（剩余2528字）