花魔芋交替氧化酶家族基因鉴定及表达分析

打开文本图片集

中图分类号：Q944.6 文献标识码：A文章编号：0439-8114（2025）12-0235-09DOI：10.14088/j.cnki.issn0439-8114.2025.12.03g开放科学（资源服务）标识码（OSID）：

Identification and expression analysis of the alternative oxidase family gene in Amorphophalluskonjac

GUANWan-ting'，HEFei，LUOChang-xin1，YIQian1，ZHAO Xiao-jie1，PUXing-yan1， SUNKe-xin1，ZHUFeng-yan1，CHUHong-long'

1.SchoolfcalddEiQugNlUvesiQuni；Cfe BiotechologygUisitiUstgingseahteicacano

Abstract：Thealternativeoxidase（AOX）familygeneinAmorphophaluskonjacK.Kochwasanalyzedatthewhole-genomelevel.The resultsshowedthat therewerefouralternativeoxidasefamilygene membersinAmorphophalushonjac，namedAkAOX1toAAOX4. Their proteinlengthrangedfrom330to361aa，molecularweightsfrom37087.52to41243.83u，nd isoelectricpointsfrom5.97to 7.27.Theywereprimarilylocated inthecytoplasmandchloroplast.TheAmorphophalus honjacalternativeoxidasefamilygnemembers were divided into two subfamilies，namelythe AOX1 subfamily（AkAOX1，AkAOX2，AkAOX3）and the AOX2 subfamily （AkAOX4）.GenecolinearityanalysisindicatednopotentialduplicatedgenepairsortandemduplicationeventsinAmopophalus konjac.Ka/Ksanalysisfurthershowedthatthesehomologousgenepairswereprimarilyunderpurifyingselectionduringevolution.The promoteregionsof theAmorpopalusonjacalterativeoxidasefamilygenecontaiedthreetypesofcisactingelements，socated with plant hormoneresponse，growthanddevelopmentresponse，andenvironmental（stress）response.TheqRT-PCRresults showed thattheexpresioleveloftheAkAOXlgeneassignificantlyup-egulatedundersoftrotpathogenifection，droughttress，andsalt stres，whileteexpresionlvelofteAAOX4gneassigficantlydowreglatedundersofrotpathogenifectio.ItwsspeculatedthattheAmorphopalluskonjacalternativeoxidasefamilygeneplayedimportantrolesinitsgrowthanddevelopment，orone signal transduction，and responses to biotic and abiotic stresses.

KeyWords：Amorphophallus konjac K.Koch；alternative oxidase（AOX）；familygene;identification；expressionanalysis

魔芋（AmorphophalluskonjacK.Koch）是中国主要栽培的魔芋品种，该物种不仅形态和生理特征独特，而且营养价值高，因此被广泛应用于食品、医药及化工等领域[1]。（剩余12409字）