白木香不同外植体愈伤组织诱导及植株再生

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关键词：白木香[Aquilaria sinensis（Lour.）Gilg.]；外植体;愈伤组织诱导;植株再生；正交试验中图分类号： 5567.1+9 文献标识码：A文章编号：0439-8114（2025）12-0228-07DOI：10.14088/j.cnki.issn0439-8114.2025.12.038开放科学（资源服务）标识码（OSID）：

Callus induction and plant regeneration from different explants of Aquilaria sinensis

YUAN Chang-ge 1，2 ， ZHOU Ya-qin³， QIN Shou-jie1，²， ZHANG Miao24，FU Peng24， TAN Xiao-ming1，2.4.5 （1.SchlofPaacyaiUeityeiciingina;aiKeybatofagdii ColaborativeovatioenterfZuangndYaeiciag3Oina;3uagiBotacalGrdeofMedialntsng 530023，Cina；4agiUestEgneResechCntefeveloptanddustlzatfZgdYal， Naning53O2ina;5.giUvesitErigseahtefoaacestcedEhnii China）

Abstract：Usingtheleafte，dnotofularensisLour.）Gilgsxprimentalaterialsnortgoaltal design was employedvia DPSsoftware.Byapplyingdiferent typesandconcentrationsof plantgrowth regulators（6-BA，2，4-D， TDZ，NAA，）tetsofothgutrsoasdcntrasoalsiutioniredo ingofAquilriasinensisreivestigatedThesultssowedtatteotialexplantforinducingAqulaiasinensicallsaeleaf， with the highest induction rate of 100% . The optimal medium for leafcallus induction was WPM+6⋅ -BA 1.5mg/L+2，4 -D 0.5mg/L+ （204号 NAA 0.8mg/L+KT0.2mg/L+TDZ1.0mg/L. Theoptimal mediumfor adventitious bud induction ofAquilaria sinensiswas WPM+6- BA 0.5 mg/L + NAA 0.2 mg/L + sucrose 20g/L+ agar 6g/L .The callus from buds and stems could differentiate into adventitious buds. The optimal medium for adventitious bud induction was WPM + 6-BA O.5 mg/L + NAA 0.2mg/L+ sucrose 20g/L+agar 6g/L ，resulting in a bud induction rate of 72.22% and an average of 5.68 adventitious buds per explant. The optimal medium for root induction was 1/2 MS + NAA 1.0mg/L+ sucrose 20g/L+ agar 6g/L ，achieving a rooting rate of 46.67% ：

Key words：Aquilaria sinensis（Lour.） Gilg.; explants；callus induction； plant regeneration；orthogonal test

白木香[Aquilariasinensis（Lour.）Gilg.]为瑞香科（Thymelaeaceae）沉香属（Aquilaria）木本植物，是《中华人民共和国药典》（2020版）收载的国产沉香药材惟一的基源植物;其主要分布于广东、云南、广西、福建和台湾等南方地区[1.2]。（剩余11068字）