湖北省1株猪链球菌9型的分离鉴定及药敏试验

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关键词：猪链球菌9型（Streptococcus suis strain9）;分离鉴定;毒力基因；致病性;药敏试验中图分类号：S855.1 文献标识码：A文章编号：0439-8114（2025）12-0166-04DOI：10.14088/j.cnki.issn0439-8114.2025.12.028开放科学（资源服务）标识码（OSID）：[

Isolation， identification and drug sensitivity test of Streptococcus suis strain 9 in Hubei Province

GUORui ∵ ，MARui 2 ，YUANFang-yan 1 ，LIUWei'，YANGKe- ⋅li1 ，LIUZe-wen1，GAOTing'，LIChang1，WUQiong'， ZHUJia-jia1，WANG Chong，TIANYong-xiang1，ZHOUDan-na1

（1.IntitutofAialHusbadndeterinarHubeicadeofluralieeKeyLboratooftreventioandotroal BacterialDiseases，theMstrofAgriculueuralAis/ubeiProvcalKeyLaboratorofLivestokadPoultryathogenicicoblog， Wuhan430064，China;2.HubeiTreGorgesPolytechnic，Yichang30，HubeiChia;3.AnimalDiseasePreventioandCotrotef CaidianDistrict，Wuhan City，Wuhan43010o，China）

Abstract：Thepathogenicbacteriawassolatedfromnurserypiglets exibitingacutefatalsymptomsofstreptococcosisatapigfamin HubeiProvince.Thepathogenwasidentifiedthroughbacterialculture，morphologicalobservation，iochemicaltests，andmolecular analysis.After inoculating clinical samples onto TSA medium supplemented with 5% sheep blood，Gram-positive cocci arranged in chainswereisolated.PCRamplificatiousingserotpe9-specificprimersandsubsequentsqueningconfirmedtheisolateasStrepto coccussuisserotye9esignatedstrainXF-1.ViulenegenescreeningrevealedtatXF-ariedfs，orf，sly，ndgapdhne， whileepfandmpgenesrenegative.Antimicrobalsuseptibilitytestinginicatedintermediatesensitivitytoefuroxiecetiofu， andampicilinutrsistanceto12antibioticsicludingcephalothinandgentamiin.Mousepathogeicitytrialsdemonstratedhigh virulence，all mice challenged with ⩾ 5 ×108 CFU died within 7 days（groups 20×108， 10×108 ，and 5×10＼$CFU），while the $1 ＼times 1 0 ^ { 8 }CFUgroupshowed40 ＼%$ mortality. Bacteria re-isolated from deceased mouse organs were PCR-confirmed as identical to XF-1.

Keywords：Streptococssuisserot9;isotiondidentificatio;vilenegne；patogenicity；ntiobalsusptibiitytet

猪链球菌（Streptococcussuis）是一种血清型多样且毒力因子复杂的革兰氏阳性病原菌，广泛定植于猪的上呼吸道（尤其是扁桃体与鼻腔）、生殖道及消化道，健康带菌猪是其主要自然宿主[12]。（剩余4754字）