一株凝结芽孢杆菌的分离鉴定及产芽孢条件优化

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关键词：凝结芽孢杆菌（Bacilluscoagulans）;分离；鉴定；芽孢；芽孢形成因子；优化

中图分类号：Q93-33 文献标识码：A

文章编号：0439-8114（2025）12-0078-06

DOI：10.14088/j.cnki.issn0439-8114.2025.12.014 开放科学（资源服务）标识码（OSID）：

Isolation and identification of a strain of Bacillus coagulans and optimizaiton of its sporulation conditions

WANGSong，WUCong，CHENYan-yan，LIJia-xin，HANYu-tong，XINGLi （JilinAgricultural ScienceandTechnologyCollege，Jilin 1321O1，Jilin，China）

Abstract：Astrainofacid-producingBacillussp.，designatedJLNKO3，wasisolatedfromthesedimentsoilfromawaterpoolinthis experiment.ThestrainasidentifiedasBaciluscoagulansthroughcolonyandcellmorphologyobservationand16SrDNAsequencing. To increase the spore production of B . coagulans strain JLNKO3，single-factor and orthogonal experiments were conducted to optimize thecultureconditionsandmediumcompositionforsporulationconditions，ndthefectsofteesporulatiopromotingfactorsonthe sporulationwerealsoinvestigated.ThersultsshowedthatheoptimalcultureconditionsforsporeproductionofstrainJLK weres follows：culture medium of 12mL ina 50 mL conical flasks，inocularatio of 3.5% （ V/V ），initial pH of 6.5，and incubation at 40‰ （204号 with shaking at 180 r/min for 50 hours. The optimal medium composition consisted of 8g/L corn starch， 14g/L soybean powder， 4g/L magnesium sulfate，and 2 g/L calcium sulfate，supplemented with 2 g/L long-chain inulin as a sporulation-promoting factor.Under this conditons，the average spore count reached（1.13±0.02） ×1010 CFU/mL，which was 3 to 4orders of magnitude higher than that beforeoptimization.Itsignificantlyincreasedthesporecount.Thismethodwasrelativelysimple，andthemediumcomponentswere cheap and easy to obtain，which could effectively reduce the cost and obtain high-yield spores.

Key Words：Bacillus coagulans； isolation； identification；spore；spore formation factor;optimization

凝结芽孢杆菌（Bacilluscoagulans）是一种既能形成芽孢，又能产乳酸的革兰氏阳性菌，具有一般乳酸菌维持肠道微生态平衡的能力，可以调节肠道功能、促进消化，所产生的抑菌物质对革兰氏阴性菌及革兰氏阳性菌均有显著的抑制效果，体现了其广泛的抗菌特性[12]。（剩余7059字）