冬凌草总黄酮超高压辅助低共熔溶剂提取及抗氧化特性

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关键词：冬凌草（Rabdosiarubescens（Hemsl.）Hara）;总黄酮;超高压；低共熔溶剂；抗氧化

中图分类号：R284.2 文献标识码：A

文章编号：0439-8114（2025）12-0181-06

DOI：10.14088/j.cnki.issn0439-8114.2025.12.031 开放科学（资源服务）标识码（OSID）：

Study on super high pressure assisted deep eutectic solvent extraction of total flavonoids from Rabdosia rubescens（Hemsl.）Hara and its antioxidant activity

WANGLe，ZHANGPei-qi²，ZONGWei²

（1.HenaIsittedaldteh；lfFd UniversityofLight Industry，Zhengzhou 45ooo2，China）

Abstract：TooptimietheextractionprocessoftotalflavonoidsfromRabdosiarubescens（Hemsl.）Harainsuperhighpressuressist eddeepeutecticsolvent（DES）andanalyzeitsantioxidantactivity，theextractionconditionwasoptimizedbysinglefactorexperimentsand response surface experiments.The scavenging activity of the extracted flavonoids against1，1-dipyl- 2 -picrylhydrazyl （DPPH）and 2，2′⋅ -azinobis（ 3- ethylbenzothiazoline 6 -ulfonic acid） diammonium salt（ABTS） free radicals was analyzed. The resultsshowedthatteoptialconditionfortheextractionof totalflavonoidsinsuperhighpressureaisteddeepeutecticsolvetwas material to liquid ratio of 1：40 （ g/mL ），water content in DESof 30% ，extraction pressure of 4O2 MPa，and extraction time of 5min， under which the yield of total flavonoids from the Rabdosia rubescens（Hemsl.）Hara was 9.21% . The half-inhibitory concentrations of the prepared total flavonoids of Rabdosia rubescens （Hemsl.）Hara forthe scavenging effects of DPPH ⋅ and ABTS ⋅⋅ were 0.136mg/mL （20 and 0.123mg/mL ，respectively.Theresearchcould providetheoreticalsupportforthedevelopmentof totalflavonoidsof Rabdosiarubescens （Hemsl.）Hara and the study of antioxidant activity.

Key words：Rabdosia rubescens （Hemsl.）Hara；totalflavonoids；superhigh pressre；deep eutectic solvent；antioxidant

冬凌草（Rabdosiarubescens（Hemsl.）Hara）为唇形科（Labiatae）植物，在中国河南、山西等地广有分布[1，2]，冬凌草中含有丰富的冬凌草素[3]、黄酮类[4]和萜类[5]等活性成分，具有抗肿瘤[6、抗氧化[7]、抗菌抗炎[和增强免疫力等功能活性，黄酮类化合物是冬凌草发挥功能作用的重要功效成分，因此，提取冬凌草中黄酮类化合物对冬凌草资源的开发具有重要作用。（剩余9624字）