广西地不容黄酮超声辅助提取工艺优化及 抗氧化性研究

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关键词：广西地不容（StephaniakwangsiensisH.S.Lo）；总黄酮;提取工艺；正交试验；抗氧化性中图分类号：R284.2 文献标识码：A文章编号：0439-8114（2025）12-0175-06DOI：10.14088/j.cnki.issn0439-8114.2025.12.030开放科学（资源服务）标识码（OSID）：

Research on optimization of ultrasonic-assisted extraction process of flavonoids from Stephania kwangsiensis H. S.Lo and its antioxidant activity

HUANGShao-jun，HUANGShi-ya，LI Tai-qiu，HUANGHao，ZHOULan，LIWan （CollegeofFoodandPhrmaceuticalEngineering，GuangxiVocationalUniversityofAgriculture，Nang53o，China）

Abstract：TooptimieeextractionprocesoftalfvonidsfromthetubersofStepaniaangsiesisH.S.Loadexploritsinvitroantioxidantactivity，asedonsinglefactorexperiments，anorthogonaltestmethodwasusedtoinvestigatethefectsofhaol concentration，solid-lqudratioextractiontemperatureandulrasonic imeontheextractionrateoftotalflavooids.Theotalextractionprocesswasetermined，andtheinvitroantioxidantactivityoftotalflvonoidsasfurtherstudied.Theresultsshowedthathe optimalextractionconditionfortotalflavonoidsfromthetubersofStephaniakwangsiensisH.S.Lowasethanolconcentrationof 70% ， solid-liquid ratio of 1：35 （ g/mL ），extraction temperatureof 50Y ，and ultrasonic timeof 5O min.Under thiscondition，theyield of total flavonoids content could reach up to 5.45mg/g . The total flavonoids from Stephania Kwangsiensis H.S.Lo exhibited strongantioxidantactivity，with the scavenging rate against 1，1 -diphenyl-2-picrylhydrazyl（DPPH） free radicalsreachingup to （84.39±0.61）% ： Inthisstudy，thultrasoundasistedextractionprocesof totalflavooidsoupledwithulravioletspectrophotometryassiplend yieldedreliablersults.Gventhattetotalflavoodspoessedgodantioxidanproperties，thismethodcouldprovideasoundeo reticalreferenceforthe extraction，development，andapplicationof totalflavonoidsfrom Stephania kuangsiensisH.S.Lo. eywords：Stephania kwangsiensis H.S.Lo;totalflavonoids；extractionprocess；orthogonaltest；antioxidantactivity

广西地不容（Stephania kwangsiensis H.S.Lo）为防己科千金藤属植物[1]，生于石山山脚土壤肥沃处或石山石缝穴中，分布于广西、贵州、云南。（剩余8085字）