转录组和代谢组联合解析水稻分藥期响应淹涝胁迫的分子机制

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关键词：转录组;代谢组;淹涝胁迫；水稻（OryzasativaL.）；分藥期；分子机制

中图分类号：S332.4 文献标识码：A

文章编号：0439-8114（2025）06-0220-12

DOI：10.14088/j.cnki.issn0439-8114.2025.06.036 开放科学（资源服务）标识码（OSID）：

Research on molecular mechanisms of Oryza sativa L. response to waterlogging stress during tillering stage using integrated transcriptome-metabolome analysis

LUO Xiao-yun 1，2 ，LI Pei-de1，²，ZHENG Xing-fei1，²，YINDe-suo1，²，WANG Hong-bo1²，HU Jian-lin1，²， HUPeng1²，LIUDan1，²，WENYi1²，CHENDong-pan 1，2 ，LEITian-jie³，XUDe-ze1，2

（1.InstituteofFodCrops/HubeiKeyLaboratoryofFoodCropGrmplasmandGeneticImprovement/KeyLaboratoryofCropMolecularBreding， Ministry of Agriculture and Rural Affairs，Hubei Academy of Agricultural Sciences，Wuhan 43oo64，China; 2.Hubei Hongshan Laboratory，Wuhan 43007o，China; 3.InstituteofEnviotandustaiableevelopmentingicultueinseademyfiulturalSices，eijioi

Abstract：UsingOryzasativaL.cultivarsEzhong6（indica）andChangligeng384（japonica）asmaterials，plantsatthetiering stage weresubjected to2/3 plantheight waterdepth treatment（lasting7days）.Transcriptomeand metabolomeanalyses were performed toinvestigatepoststressgeneexpresionandmetabolitechanges.Tanscriptomeanalysisidentied38and1624dieren tiallyexpressedgenes（DEGs）inEzhongandChangligeng384，espectively，whichweresignificantlyenrichedincarbohratebio syntheticprocess，eactiveoxygenspeciesmetabolicprocss，lantpathogeninteractio，ndplanthonesignal ransductipath ways.Metabolomeanalysisrevealedsignificantenrichmentofdiferentiallaccumulatedmetabolites（DAMs）suchasbenzeneandsubstitutedderivativesrganicacids，andlactonsinEzhong6ndChangligeng384，primarilyinvolvedinditerpenoidbiosythsis flavonoidbiosynthesispathways.Integratedtranscriptome-metaboomeanalysisidentifiedsevencoenrichedpathwaysinbothcultivars：Metabolicpathways，ioytsisofscondarymetaboites，iosytesisofiousplantsecondarytaboites，iterpeidbi synthesis，oleiccidetabolisnlpropaidosythsis，ndcarotenidsytsisOrasatiaLspondedtatel ging stressbyactivating antioxidant systems，regulating hormone signaling，and accumulating defensive metabolites.

KeyWords：transcriptome；metabolome；waterloggingstress；ice（OryzasatiaL.）；tillringstagemolecularmechanisms

收稿日期：2025-04-29

随着全球人口持续增长、耕地资源逐年减少以及自然灾害频繁发生，粮食安全问题已成为21世纪人类面临的重大挑战。（剩余20315字）