基于生物信息学的掌叶覆盆子matK基因多样性分析

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关键词：生物信息学；掌叶覆盆子（RubuschingiiHu）； matK 基因；多样性分析

中图分类号：S567.19 文献标识码：A

文章编号：0439-8114（2025）06-0215-05

DOI：10.14088/j.cnki.issn0439-8114.2025.06.035 开放科学（资源服务）标识码（OSID）：

Diversityanalysis of matK gene in Rubuschingii Hu based onbioinformatics

YUANLi-xia，ZHOULei，LOUTian-ling，WANGFu-rong，WUHao （Colege ofPharmaceutical Engineeringand Biotechnology/Collgeof Cosmetics，Zhejiang Pharmaceutical University， Ningbo ，Zhejiang，China）

Abstract：Usingbioinformaticsmethods，multi-dimensionalanalysiswasperformedonthechloroplastmatKgeneof Rubuschingi Hu，ncludingopenreadingframeprediction，minoacidompositionandpysicochemicalpropertyanalysis，hydrophilicitydro phobicityassessment，signal peptide prediction，subcellarlocalization，andspatial structure modeling.Meanwhile，phylogenetic analysisand evolutionary treeconstruction wereconductedfor14 speciesbasedonmatK genesequences.Theresultsshowed that thematKgeneof Rubuschingi Huwas1512bpinlengthandencoded503aminoacids.ThematKproteinwaslocalizedinchloroplasts with 95% confidencelevel，hadanisoelectric pointof9.73（indicatinganalkaline protein），andshowedan instabilityindexof 42.52andahdropliyoeffcetof-052，lasifingitsnunsabledrophilicprotenNosigalpeptidwasdetedinthe matK protein.The secondary structure of matK protein primarily consisted of random coils （246 residues）， α -helices（171 residues）， and extended strands （86 residues），with random coils accounting for the highest proportion （ 48.90% ）and extended strands the lowest （ 17.10% ）. The tertiary structure achieved a GMQE score of O.61 with 93.64% sequence similarity.Phylogenetic analysis based onmatKgenesequencesrevealedthatRubuschingii Huand13otherspecieswereclassifiedinto threeclusters.Rubuschingi HuformedacladewithRubusrosifolius，Rubusursinus，andRubus elipticus，howingtheclosestgeneticrelationshipwithRubusrosifolius.

Keywords：bioinformatics；Rubus chingii Hu；matK gene；diversity analysis

掌叶覆盆子（RubuschingiiHu）作为蔷薇科悬钩子属植物，具有较高的药用价值和经济价值。（剩余6917字）