野桑蚕全长转录组测序及生物信息学分析

打开文本图片集

关键词：野桑蚕（Bombyxmandarina）；全长转录组；测序；生物信息学分析

中图分类号：Q966；S881.2 文献标识码：A

文章编号：0439-8114（2025）06-0197-10

DOI：10.14088/j.cnki.issn0439-8114.2025.06.033 开放科学（资源服务）标识码（OSID）：

Full-length transcriptome sequencing and bioinformatics analysis of the Bombyx mandarina

MENG Gang，WANG Rui-xian，CHU Qu，PENG Yun-wu，YANG Jin-hong， CHEN An-li， ZHANG Sheng-yuan，LING Jun （Shaanxi Key Laboratory of Sericulture，Ankang University，Ankang 725oOO，Shaanxi，China）

Abstract：Thisstudyemployedsecond-generationsequencing（lluminaRNA-Seq）tocalibrate third-generationsequencing （PacBioISO-Seq）forconductingful-length transcriptomesequencingoftheBombyx mandarina，aimingtoinvestigategnexpres sioncharacteristsduringdiferentpupaldiapauseanddeeplyexploregenomicfunctionalinformatio.Atotalof93616fullength transcripts wereobtainedthroughsequencingandassembly，withasequencelengthof327～3273bp，anaveragelengthof2631bp， andN50of3204bp.IntegrationofannotationsfromCOG，GO，KEGG，KOG，Pfam，SwissProt，eggNOG，andNRdatabasesidentified82796functionallyannotatedgenes.Analysisoffullength transcriptsrevealed17189lncRNAs，87921SSRmarkersand 49432openreading frames（ORFs），withORF-encoded proteinsrangingfromOto1522aminoacids（average305）.Gene expressionanalysisacrossdiapausestagesidentified578diferentiallyexpressedgenes，including269withGotationsand1590 with KEGG annotations.

Key Words：Bombyx mandarina； full-length transcriptome；sequencing；bioinformatics analysis

野桑蚕（Bombyxmandarina）是一种主食桑叶的鳞翅目蚕蛾科野生绢丝昆虫。（剩余18060字）