嗜黏蛋白阿克曼菌对大肠杆菌K88刺激的小鼠回肠凋亡和自噬信号通路相关基因表达的影响

打开文本图片集

关键词：产肠毒素大肠杆菌K88；嗜黏蛋白阿克曼菌；回肠；凋亡；自噬

中图分类号：S852.61 文献标识码：A

文章编号：0439-8114（2025）06-0141-05

DOI：10.14088/j.cnki.issn0439-8114.2025.06.023 开放科学（资源服务）标识码（OSID）：

The effect of Akkermansia muciniphila on ileal apoptosis and autophagy signaling pathway related gene expression in mice stimulated by Escherichia coli K88

ZHANGZi-han，LIULi-xue，HUJin （HubeiKeyLaboratoryof AnimalNutritionandFeed Science，WuhanPolytechnic University，Wuhan43oo23，China）

Abstract：ToinvestigatetheefectofAkermansiamuciniphila（AKK）ontheexpresionof genesrelatedtoapoptosisandautophagy signalingpathwaysineiluaftertoxinogenicEscherichcoli（ETEC）K88stiulationiducesintestialinjury8eaeBB/c mice（5～6 weksold，weighing18±1g）wererandomlydivided into 3treatmentgroups，each treatmentwith6replicates.After1 week of normalfeeding，themicewereorallygavagedwithPBS，liveAKKbacteria，orheat-inactivatedAKKbacteria.Thegavage wasperformedonceevery2d，andafterweksofcontiuousgvage，ETECK88bacterialsolutionasintraperitoneallinjctedfor infection，andfter36h，themicewereanestheizedandsacrificed，andintestialsampleswereretrieedtoetecteeprssionof genes elatedtoapoptosisandautophagysignalingpathway.Theresultsshowedasfolows：（1）AKKbacteriaandinactivated AKK bacteriahadalleviatingeffectsonthedamageof ilealmorphologyandstructurecausedbyETECK88infection.（2）Bothgavageand inactivated AKK significantly down-regulated the mRNA expression of interleukin IL- 1β ，IL-6 and tumor necrosis factor- α （TNFα ）in the ileum of mice（ P<0.05 ）.（3）AKK significantly reduced the mRNA expression levels of Fasl， Caspase 3，Caspase 9 and Bcl- ⋅2⋅ related X protein（Bax），which are related to the apoptosis signaling pathway in mice，（ P<0.05 ）.（4）AKK bacteria or inactivatedAKKbacteriasignificantlyeducedtheRNAexpresionlevelsofmammalianlepatoxintargetprotein（mTOR），andautophgy-related gene 12（Atg12）in the ileum of mice （ P<0.05 ）.AKK bacteria has a certain alleviating effect on ileal injury in mice caused by ETEC K88 infection.

Key words： toxinogenic; Escherichia coli K88；Akkermansia muciniphila; ileum； apoptosis；autophagy

产肠毒素大肠杆菌（Enterotoxigenic Escherichiacoli，ETEC）是导致动物尤其是幼畜腹泻的主要致病菌。（剩余8789字）