不同樱桃番茄品种2-苯乙醇及其糖苷含量与相关基因的表达

打开文本图片集

关键词：樱桃番茄;2-苯乙醇;糖苷;2-苯基乙基β-D-葡萄糖苷;含量;基因表达

中图分类号：S641.2；Q943.2 文献标识码：A

文章编号：0439-8114（2025）06-0207-08

DOI：10.14088/j.cnki.issn0439-8114.2025.06.034 开放科学（资源服务）标识码（OSID）：I

Content of 2-Phenylethanol and its glycosides and expression of associated genes in different cherry tomato cultivars

SHI Xian-peng1，SONG Min1，WU Cheng-yu1，LIULi-feng²，CHENG Fei1，MA Jing1 （1.CollgeofortiltureEgneigLabratoryfenetiImprovementofHrtiluralCosofSadogroveQgdaKebtoyf Genetic ImprovementandBredinginHorticulturalPlants，QingdaoAgriculturalUniversity，Qingdao266109，Shandong，hina; 2.Institute of Vegetables，Shandong Academy of Agricultural Sciences，Jinan 25O1OO，China）

Abstract：Fourcherrytomato（Solanum lyopersicumL.）cultivars（Qianxi，Xiaohong，T7，andFushan88）wereusedasexperiental materials.The contents of 2-phenylethanol and 2-phenylethyl β -D-glucoside in fruits were determined by ultra-performance liquidchromatographycoupled withquadrupole time-of-flighttandemmasspectrometry（UPLC-QTOF-MS/MS）.Potentialuridine diphosphate glycosyltransferase（UGT）genes werescreened through phylogeneticanalysis.Theirexpressionpaterns were analyzed by reverse transcriptionquantitative polymerasechainreaction（RT-qPCR）to investigate thecorelation between thecontentsof 2- phenylethanol/ 2⋅ phenylethyl β-D-glucoside andrelated gene expression levels.The results showed significant diferences in 2- （204号 phenylethanol and 2-phenylethyl β -D-glucoside contents among cultivars，with the order of Qianxi>Xiaohong>T7 > Fushan 88.The highest contents of 2-phenylethanol（3 906.69 ng/g ）and2-phenylethyl β -D-glucoside（5 590.26 ng/g）were detected in Qianxi， bothsignificantlyhigherthanthoseinothercultivars.Phylogeneticanalysisidentified2OSlUGTgenes.Amongthem，SlUGT42， SUUGT54，SlUGT103，SlUGT104，and SlUGT105showed increasing expresion trends during fruit development.SlUGT103， SUUGT104，andSUGO5exhibitedthehighestexpresionlevelsinQianxi，coincidingwithitspeak2-phenlethanolandglcoside contents.TherelativeexpresionofSUGO3showedsignificantpositivecorrelationswithboth2-phenylethanoland2-pyletyl β-D-glucosidecontets.TherelativeexpressoofSUGO4wassgnificantlypositivelycorelatedwith2-phenylethanolontent. SlUGT103and SlUGT1O4 wereidentifiedaskeyglucosyltransferase genes catalyzingtheglycosylationof2-phenylethanol.

KeyWords：chery tomato；2-phenylethanol;glycoside；2-phenylethylβ-d-glucoside；content;gene expression

番茄（SolanumlycopersicumL.）为茄科番茄属的1年生草本植物，是世界范围内广泛种植的蔬菜作物，是三大世界性贸易蔬菜之一[1]，在中国农业生产中有着举足轻重的地位[2]。（剩余11594字）